Description Estrogen Receptor beta  (ESR2) belongs to the nuclear hormone receptor family. ESR2 binds estrogens with high affinity, similar to Estrogen Receptor alpha (ESR1). At least 8 isoforms of ESR2 are produced by alternate splicing.
Batch No. See product label
Unit size 100 µg
Antigen A synthetic peptide (HGSIYIPSSYVDSHHEYPAMTFYS) corresponding to a region (27-50) from human Estrogen Receptor beta. To enhance the immunological response, this peptide was coupled to carrier protein BSA.
Other Names ER-beta; Nuclear receptor subfamily 3 group A member 2; ESR2; ESTRB; NR3A2;
Accession Q92731 ESR2_HUMAN;
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Western Blotting (WB). A concentration of 0.5-1.0 µg/ml is recommended for WB. Human Estrogen Receptor beta (isoform Beta-1) has a predicted length of 530 residues and MW of 59 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB against the antigen.
Cross-reactivity Human. The antibody is predicted to react with rat due to sequence homology.
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule. The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please choose carefully, as the sequence you choose may be a precursor rather than the mature protein for example.
Form Lyophilised with 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Reconstitution Reconstitute in 100 µl of sterile distilled water to achieve an antibody concentration of 1 mg/ml. Centrifuge to remove any insoluble material.
Storage At least 12 months after purchase at 2 - 4°C (lyophilized formulations).After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Expiry Date 12 months after purchase