Description A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighbouring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-26 belongs to the class 1 (beta) group of connexins. Mutations in Connexin-26 are associated with genetically derived hearing impairments, including autosomal recessive non syndromic deafness.
Batch No. See product label
Unit size 100 µg
Antigen A synthetic peptide (CYLFIRYCSGKSKRPV) corresponding to the amino acid sequence 211-226 from rat Connexin-26.
Other Names GJB2; Cx26; Gap junction beta-2 protein;
Accession P21994 CXB2_RAT; P29033 CXB2_HUMAN
Produced in Rabbit
Purity Affinity purified on antigen column
Applications Western Blotting (WB). A concentration of 1.0-2.0 µg/ml is recommended for WB. The predicted length of rat Connexin-26 is 226 residues and MW of 26 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Specificity The specificity of this antibody has been confirmed by WB against the antigen.
Cross-reactivity Human; mouse; rat; rabbit;
Blast it If you wish to use this product in a species other than those specified here and want to determine the amino acid sequence homology between the immunogen and the corresponding protein in that species, simply copy the amino acid sequence for this immunogen and test it on the BLAST database that you can access through the link below. Please note that antibodies raised against synthetic peptides are quite often very specific for that peptide. What this means in practise is that a single amino acid difference may be enough to restrict the specificity to a particular molecule.The BLAST database can also be used to compare any amino acid sequence between species and between protein. The full amino acid sequence of the target protein can be found by following the link in the Accession field. You can then search any amino acid sequence in the target protein using the BLAST database. When selecting a sequence to search, please chose carefully, as the sequence you chose may be a precursor rather than the mature protein for example.
Form Liquid (0.5ml). 50% glycerol, 0.9mg NaCl and 0.2mg Na2HPO4
Reconstitution The liquid formulation should be diluted in PBS (pH 7.4)
Storage After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Expiry Date 12 months after purchase