Description | Nervegrowthfactor(NGF)receptor,alsoknownasp75NTR, isalowaffinityNGFreceptor.ItbindswithequalaffinityneurotrophinssuchasbetaNGF,BDNF,NT-3andNT-4.NGFreceptorsmediatesignalingofneurotrophinsforneuronalsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.ThesereceptorsarealsothoughttoplayaroleinneurogenerativediseasessuchasAlzheimersdisease.TheNGFreceptorisatypeItransmembraneglycoprotein(399aa)consistingofasignalpeptide(28aa),anextracellulardomain(222aa)whichcontainsfourcysteinerichdomainsresponsIBLeforligandbinding,atransmembranedomain(22aa)andacytoplasmicdomain(155aa). |
BatchNo. | Seeproductlabel |
Unitsize | 25µg |
OtherNames | Low-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR;p75NTR;CD271; |
Accession | P08138TNR16_HUMAN; |
Producedin | Human-ADNAsequenceencodingthesignalpeptideandextracellulardomainofhumanNGFreceptor(aminoacids1-250)wasfusedtotheFcregionofhumanIgG1(aminoacids93-330).Thechimericproteinwasexpressedinmodifiedhuman293cells. |
MolecularWeight | TheNGFReceptor-Fcchimeramigratesasabroadbandbetween75and100kDainSDS-PAGEduetopost-translationmodifications,inparticularglycosylation.TheunmodifiedNGFReceptor-Fcchimerahasapredictedmassofof50.8kDa.TheNGFReceptor-FcchimeraseparatesintoanumberofisoformswithapIbetween4.0and6.0in2DPAGE.TheunmodifiedNGFReceptor-FcchimerahasapredictedpIof4.89. |
Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
Biol.activity | TheED50oftheNGFReceptor-Fcchimeraistypically0.7-1.0ug/mlasmeasuredbyitsABIlitytoneutralizebetaNGFmediatedproliferationofthehumangrowth-factordependentTF-1cellline. |
Form | TheNGFReceptor-Fcchimeraconsistsof30-50%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,andlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。