Description | Saporinisaribosome-inactivatingprotein(RIP)oftypeI.ThismonomericRNAN-glycosidasepurifiedfromseedsoftheplantSaponariaofficinalisalsoknownasSoapwort,iscapableofspecificdepurinationofeukaryoticribosomesthusarrestingproteinsynthesis.NoligandhasbeenidentifiedinsaporinhenceitsinABIlitytotransversethecellmembrane.Duetoitstoxicityandstabilityofthestructure,saporinhasprovenextremelyusefulforconstructionofimmunotoxins. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
OtherNames | Saponariaofficinalis;Commonsoapwort;ribosomeinactivatingproteinsaporin-6;SAP-6;SO-6;rRNAN-glycosidase |
Accession | RIP6_SAPOF |
Producedin | PurifiedfromseedsoftheplantSaponariaofficinalis. |
MolecularWeight | 29.5kDa |
Handling | Onlyqualifiedpersonnelshouldhandlethisproduct.Extremeprecautionmustbeemployedwhenhandlingthetoxin.Wearlabcoat,glovesandsafetyglasses.Thetoxinmustnotcomeintocontactwithopenwounds.Practicegoodlaboratorytechniquetoavoidauto-exposure/auto-injection!Washthoroughlyanypartofthebodyexposedtothetoxin.Contaminatedmaterialsshouldbeexposedto300mMNaOHorautoclaved. |
Purity | 95%bySDS-PAGEandWesternBlot. |
Biol.activity | Uponintroductionintothecells,saporinwillarresttheproteinsynthesisbydamagingtheribosmes. |
Form | Lyophilised |
Reconstitution | SaporinmaybereconstitutedinthebufferofchoiceforexamplePBS. |
Storage | Aliquotandkeepat-20ºCforlong-termstorage.Forshorttermkeepat4ºC.Avoidrepetitivefreeze/thawcycles. |
References | Stirpe,F.etal(1983)BiochemJ216,617-625 |
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