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主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain for identifying Normal & Path
产品编号:TR-100-BG
市  场 价:¥11940.00
场      地:美国(厂家直采)
产品分类: 生化试剂>其他>其他试剂>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$597.00
品      牌: Biosensis
公      司:Biosensis
公司分类:
Biosensis/Black-Gold II Myelin Ready-to-Dilute Staining Kit with Toluidine Blue O Counter Stain for identifying Normal & Path
商品介绍
DescriptionBlack-Gold®IIisanovelhaloaurophosphatecomplexwhichlocalisesmyelinwithinthecentralnervoussystem.TheBlackGoldIIReady-to-Dilute(RTD™)StainingKitallowsyoutolocalisemyelin,bothindividualfibresandtracts,alongwiththeoptionofco-localisingcellbodiesviatheToluidineBluecounterstain.BlackGoldIIlabelledmyelinatedfibresappearnearlyblackwhiletheToluidineBlueOlabelledcellularNisslbodiesareblueunderbrightfieldIllumination.

BlackGoldIIcandemonstrateandcharacterisespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,domoicacid,3-nitropropionicacid,Fluoro-Goldandisoniazid.BlackGoldIIcanalsobecombinedwithotherhistochemicalMarkersincludingNisslstains,retrogradelytransportedfluorescenttracersandfluorescentmarkersofneuronaldegeneration.TheadvantagesassociatedwiththeBlack-GoldIIincludehighresolution,highcontrast,shorthistochemicalprocessingtime,versatilityandconsistentreproducibility.
OtherNamesblackandgold
ApplicationsBlackGoldIIisahighresolutionmyelinstainwithamyloidplaquecounterstain.Itsuseistailoredtostudiesusingformalinorparaformaldehydefixed,non-paraffinembedded,non-solventprocessedbraintissue.Itcanbeusedwithboththickandthinsections.Forthicksections,gelatincoatedslidesorslidesspeciallydesignedtobindtissuessectionsshouldbeusetoavoidsectionloss.Free-floatingsectionscanbeusedaswellbutsectionsareeasiertohandleandtransferwhenmountedonslides.Asuggestedmethodforthicksectionsisprovidedasaguide:Eitherfrozenorvibratomesectionsarecutatathicknessof20-50umandcollectedin0.1Mneutralphosphatebuffer.Thesectionsarethentypicallymountedon1%gel-coatedslidesandthenairdriedonaslidewarmer(at50degreesC)foratleastanhouruntilthroughlydriedandadheredtotheslide.Thesectionscanbestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.Themountedsectionswererehydratedindistilledwaterfor2minutesbeforebeingprocessedinthestainingsolutions.
FormThereagentsintheBlackGoldkitareallsuppliedinaliquidformatandareready-to-dilute.
StorageThekitcanbetransportedatroomtemperature.Oncereceived,thekitcanbestoredforupto12monthsat4°Cprotectedfromlight.Dilutedsolutionscanbestoreduptoonemonthat4°Cprotectedfromlight.
SpecificReferencesYingYLetal.(2014)Adultneuralprecursorcellsfromthesubventricularzonecontributesignificantlytooligodendrocyteregenerationandremyelination.JNeurosci.2014Oct15;34(42):14128-46
KitComponentsBlack-GoldII(Dilute1:10priortouse)-10mLSodiumThiosulfate,fixative(Dilute1:10priortouse)-10mLToluidineBlueO(Dilute1:10priortouse)-10mLAceticAcid(Dilute1:10priortouse)-10mL
ReagentKitprotocolClick to download the protocolTR-100-BG_April2012.pdf
MSDSClick to download the Material Safety Data SheetMSDS_TR-100-BG_BGT_as_at_Apr2014.pdf
品牌介绍

Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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