Description | TrkAisamemberoftheneurotrophictyrosinekinasereceptorfamily.Itisamembrane-boundreceptorthatuponneurotrophinbinding,phosphorylatesitselfandmembersoftheMAPKpathway.TrkAisrequiredforhigh-affinitybindingtonervegrowthfactor(NGF),neurotrophin-3andneurotrophin-4/5butnotbrain-derivedneurotrophicfactor(BDNF).TrkAleadstocelldifferentiationsandmayplayaroleinspecifyingsensoryneuronsubtypes.Ithasacrucialroleinthedevelopmentandfunctionofthenociceptivereceptionsystemaswellasestablishmentofthermalregulationviasweating.SUBUNIT:Existsinadynamicequilibriumbetweenmonomeric(lowaffinity)anddimeric(highaffinity)structures.SUBCELLULARLOCATION:TrkAisaheavilyglycosylatedtransmembraneproteinandcontains13potentialN-linkedglycosylationsites.TheTrkA-FcchimerahasN-linkedandmayhaveO-linkedoligosaccharides. |
BatchNo. | Seeproductlabel |
Unitsize | 50µg |
OtherNames | Tropomyosin-relatedkinasereceptor;Highaffinitynervegrowthfactorreceptor;Neurotrophictyrosinekinasereceptortype1;TRK1transformingtyrosinekinaseprotein;p140-TrkA;Trk-A;NTRK1;TRK |
Accession | P04629NTRK1_HUMAN; |
Producedin | Human-ADNAsequenceencodingthesignalpeptideandextracellulardomainofhumanTrkA(aminoacids1-413)wasfusedtotheFcregionofhumanIgG1(aminoacids104-330).Thechimericproteinwasexpressedinmodifiedhuman293cells. |
MolecularWeight | TheTrkA-Fcchimeramigratesasabroadbandbetween90and120kDainSDS-PAGEunderreducingconditionsduetopost-translationalmodifications,inparticularglycosylation.TheunmodifiedTrkA-Fcchimerathathasapredictedmolecularmassof70.2kDaandpredictedpIof5.8.Undernon-reducingconditions,TrkA-Fcmigratesasabroadbandbetween180and240kDademonstratingtheexistenceofthisproteinasadimer.Asecond,lessintensebandisobservedat140kDaundertheseSDS-PAGEconditions,suggestingthepresenceofnon-glycosylatedTrkA-Fcisoforms. |
Purity | >95%,asdeterminedbySDS-PAGE. |
Form | ProteinGpurified. |
Reconstitution | Spinvialbrieflybeforeopening.Reconstitutein100uLsterilePBS,pH7.4togiveaconcentrationof0.5mg/mL. |
Storage | Lyophilizedproductshouldbestoredat2to8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
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