Description | StemCellFactorReceptor(SCFR)isamemberofthereceptortyrosinekinasefamily.BindingofSCFRtoitsligandStemCellFactor(PE-2008-5)inducesreceptordimerization,autophosphorylationandsignaltransduction.SCFRisessentialforthedevelopmentofnormalhematopoieticcellsandplaysanimportantroleinthesurvival,proliferationanddifferentiationofmastcells,melanocytesandgermcells. |
BatchNo. | Seeproductlabel |
Unitsize | 50µg |
OtherNames | Mast/stemcellgrowthfactorreceptor;SCFR;Proto-oncogenetyrosine-proteinkinaseKit;c-kit;CD117 |
Accession | P10721KIT_HUMAN; |
Producedin | Human-ADNAsequenceencodingthesignalpeptideandextracellulardomainofhumanStemCellFactorReceptor(aminoacids1-520)wasfusedtotheFcregionofhumanIgG1(aminoacids93-330).Thechimericproteinwasexpressedinmodifiedhuman293cells. |
MolecularWeight | TheSCFR-FcChimeramigratesasabroadbandbetween90and150kDainSDS-PAGEduetopost-translationalmodifications.ThiscompareswiththeunmodifiedSCFR-FcChimerathathasapredictedmolecularmassof83.1kDa.TheChimeraseparatesintoanumberofisoformswithapIbetween4.5and8in2DPAGEduetopost-translationalmodifications.TheunmodifiedSCFRFcChimerahasapredictedpIof6.6. |
Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
Biol.activity | TheED50oftheSCFR-FcChimeraistypically1-3ug/mlasmeasuredbyitsABIlitytoneutralizeSCFmediatedproliferationusingthehumangrowthfactordependentM-07ecellline. |
Form | TheSCFR-FcChimeraconsistsof5-45%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,andlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
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