Description | L-selection(CD26L)isacellsurfaceadhesionproteinconstitutivelyexpressedonallclassesof humanleukocytes. TheL-SelectinglycoproteiniscomposedofaC-typelectinfamilydomainattheaminoterminus,anEGF-likedomain,twoshortconsensusrepeat(SCR)sequences,atransmembranedomainandashortcytoplasmictail.L-Selectincontains7potentialN-linkedglycosylationsites. |
BatchNo. | Seeproductlabel |
Unitsize | 50µg |
OtherNames | Lymphnodehomingreceptor;Leukocyteadhesionmolecule1;LAM-1;LeukocytesurfaceantigenLeu-8;TQ1;gp90-MEL;Leukocyte-endothelialcelladhesionmolecule1;LECAM1;CD62L;SELL;LNHR;LYAM1; |
Accession | P14151LYAM1_HUMAN; |
Producedin | Human-ADNAsequenceencodingthesignalpeptide,pro-peptideandextracellulardomainofhumanL-Selectin(aminoacids1-343)wasfusedtotheFcregionofhumanIgG1(aminoacids93-330).Thechimericproteinwasexpressedinmodifiedhuman293cells. |
MolecularWeight | TheL-Selectin-Fcchimeramigratesasabroadbandbetween75and105kDainSDS-PAGEduetopost-translationmodifications,inparticularglycosylation.TheunmodifiedL-Selectin-Fcchimerahasapredictedmolecularmassof59.9kDa.TheL-Selectin-FcchimeraseparatesintoanumberofisoformswithapIbetween4.6and6.5in2DPAGE.TheunmodifiedL-Selectin-FcchimerahasapredictedpIof5.92. |
Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
Form | TheL-Selectin-Fcchimeraconsistsof20-45%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
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