Description | EpidermalGrowthFactor(EGF)ReceptorbindstoEGFandothermembersoftheEGFfamilysuchasTGF-alpha,amphiregulin,betacellulin,heparin-bindingEGF-likegrowthfactor,GP30andvacciniavirusgrowthfactor.Atleast4isoformsareproducedbyalternativesplicing(referenceSWISSPROT).TheEGFreceptorisasingle-passtypeImembraneproteincontaininganextracellularreceptordomain,atransmembraneregionandanintracellulardomainwithtyrosinekinasefunction. |
BatchNo. | Seeproductlabel |
Unitsize | 25µg |
OtherNames | Receptortyrosine-proteinkinaseerbB-1;EGFR;Urogastrone;Pro-epidermalgrowthfactor;EGFR;c-erbB-1;ERBB1; |
Accession | P00533EGFR_HUMAN; |
Producedin | Human-ADNAsequenceencodingthesignalpeptideandextracellulardomainofhumanEpidermalGrowthFactorReceptor(aminoacids1-525)wasfusedtotheFcregionofhumanIgG1(aminoacids90-330).Thechimericproteinwasexpressedinmodifiedhuman293cells. |
MolecularWeight | Underreducingconditions,theEGFreceptor-Fcchimeramigratesasabroadbandbetween85and110kDaonSDS-PAGEduetopost-translationalmodifications,inparticularglycosylation.TheunmodifiedEGFreceptor-Fcchimerahasapredictedmonomericmolecularmassof83kDa.ThechimeraseparatesintoanumberofglycoformswithapIbetween6.0and8.5on2DPAGE.TheunmodifiedchimerahasapredictedpIof7.8. |
Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbyCoomassieBrilliantBlue |
Biol.activity | TheED50oftheEGFreceptor-Fcchimeraistypically60-100ng/mlasmeasuredbyitsABIlitytoneutralizeEGFmediatedproliferationofmurineNIH3T3fribroblasts. |
Form | TheEGFreceptor-Fcchimeraconsistsof5-30%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,withlonger-termstorageinaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
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