Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | TheratTrkAKitisasandwichELISA.ThecaptureantibodyisapolyclonalratTrkAantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.TestsamplesandstandardsofknownTrkAconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundTrkAantibody.Abiotinylatedratpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofTrkApresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofratTrkAinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | Antigen | TrkAisamemberoftheneurotrophictyrosinekinasereceptorfamily.Itisamembrane-boundreceptorthatuponneurotrophinbinding,phosphorylatesitselfandmembersoftheMAPKpathway.TrkAisrequiredforhigh-affinitybindingtonervegrowthfactor(NGF),neurotrophin-3andneurotrophin-4/5butnotbrain-derivedneurotrophicfactor(BDNF).TrkAleadstocelldifferentiationsandmayplayaroleinspecifyingsensoryneuronsubtypes.Ithasacrucialroleinthedevelopmentandfunctionofthenociceptivereceptionsystemaswellasestablishmentofthermalregulationviasweating. | OtherNames | Tropomyosin-relatedkinasereceptor;Highaffinitynervegrowthfactorreceptor;Neurotrophictyrosinekinasereceptortype1;TRK1transformingtyrosinekinaseprotein;p140-TrkA;Trk-A;NTRK1;TRK | Accession | P35739NTRK1_RAT; | Specificity | TrkA | Storage | Storeat4°C | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 156pg/ml-10,000pg/ml | Sensitivity | < 10pg/ml | Kitprotocol | BEK-2201-2P_as_at_Nov2013_protocol.pdf | 品牌介绍
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