Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | TheratTNF-alphaKitisasandwichELISA.ThecaptureantibodyisamonoclonalratTNF-alphaantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.RattestsamplesandstandardsofknownTNF-alphaconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundTNF-alphaantibody.AbiotinylatedratTNF-alphapolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofTNF-alphapresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofratTNF-alphainsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | BatchNo. | Seeproductlabels | Antigen | Tumornecrosisfactoralpha(TNF-alpha)isacytokinethatbelongstothetumornecrosisfactor(TNF)superfamily.TNF-alphaismainlysecretedbymacrophages.TNF-alphaisinvolvedintheregulationofmanyprocessessuchas�cellproliferation,differentiationandapoptosisandhasbeenimplicatedinavarietyofdiseasesincludingcancer.TNF-alphaisexpressedasasingle-passtypeIImembraneprotein.Asoluble,secretedformisderivedfromthemembraneformbyproteolyticprocessing. | OtherNames | TNF-alpha;Tumornecrosisfactorligandsuperfamilymember2;TNF-a;Cachectin;TNF;TNFA;TNFSF2; | Accession | P16599TNFA_RAT; | Specificity | RatTNF-alpha | Storage | Storeat4°C | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 15.6pg/ml-1,000pg/mlforbodyfluids,tissuelysatesorcellculturesupernates.7.8pg/ml-500pg/mlforratsera. | Sensitivity | < 1 pg/ml | Kitprotocol | BEK-2101-2P_as_at_Jun2017_protocol.pdf | 品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。
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