Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | ForquantitativedetectionofratHGFincellculturesupernatants,serum,andcitrate,orEDTAtreatedplasmasamplesonlywhenusedasdirected | TheratHepatocyteGrowthFactor(HGF)KitisasandwichELISA.ThecaptureantibodyisamonoclonalmouseHGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(alpha)andV496-L728(beta))andtestsamplesareaddedtothewells.Abiotinylateddetectionpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofHGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseHGFinsamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Range:156pg/mL-10,000pg/mL;Sensitivity<10 pg/mL BatchNo. | Seeproductlabels | Antigen | Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(alpha)andV496-L728(beta)). | Sequence | Immunogensequence:aminoacids33-495foralphachain,andaminoacids496-728forthebetachainofHGF | OtherNames | Scatterfactor;Hepatopoeitin-A;SF; | Accession | P17945HGF_RAT; | Applications | ELISA | Specificity | NaturalandrecombinantRatHGF | Cross-reactivity | Thereisnodetectablecross-reactivitywithotherrelevantproteins. | ExpiryDate | Unopenedkitstableat4˚Cfor6months,at-20˚Cfor12months. | Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 156pg/mL-10,000pg/mL | Sensitivity | < 10 pg/mL | Kitprotocol | Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit. | 品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。
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