Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com | |
Description | The mouse Transforming growth factor beta-1 (TGFb1) Kit is a sandwich ELISA. The capture antibody is a monoclonal TGFb1 antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known TGFb1 concentration are added to these wells and allowed to complex with the bound TGFb1 antibody. A biotinylated TGFb1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TGFb1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse TGFb1 in cell culture supernatants, serum, plasma (EDTA treated samples only) and urine. Acid pretreatment is required for best activity. Free TGFB1 is high labile. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
Batch No. | See product labels |
Other Names | TGFB1; TGFB; TGF-beta-1; |
Accession | P04202 TGFB1_MOUSE; |
Specificity | Human; Mouse; Rat; |
Cross-reactivity | The cross reactivity with TGFβ2, TGFβ3 and TGFβ5 is < 1%. |
Storage | Store at 4°C |
Kit components | The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
Range | 15.6 pg/ml - 1,000 pg/ml |
Sensitivity | < 2 pg/ml |
Kit protocol | BEK-2095-2P_as_at_Jun2017_protocol.pdf |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。