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℡ 4000-520-616
℡ 4000-520-616
Biosensis/Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (1 Plate)/BEK-2218-1P/1 Ea
产品编号:BEK-2218-1P
市  场 价:¥7960.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$398.00
品      牌: Biosensis
公      司:Biosensis
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Biosensis/Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (1 Plate)/BEK-2218-1P/1 Ea
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Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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DescriptionTheBiosensisNeurotrophin4/5RapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthespecific,fastandreliablequantificationofNT4/5inlessthan4hoursincellculturesupernatants,humancitrate-plasmaandbrainextractsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularbloodsamples.AccuratequantificationofNT4/5inhumancitrate-plasmarequiresaheterophilicantibody(HA)blockerwhichcanbepurchasedseparately(BL-003-1000).

ThisELISAkitconsistsofapre-coatedpolyclonalanti-NT4/5captureantibody,abiotinylatedanti-NT4/5detectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3’,5,5’-tetramethylbenzidine,TMB)yieldsacoloredreactionproduct,whichisdirectlyproportionaltotheconcentrationofNT4/5presentinsamplesandproteinstandards.

ThisNT4/5ELISAkitemploysarecombinanthumanNT4/5standard.ThecaptureanddetectionantibodieswillalsodetectNT4/5fromotherspeciesduetoahighdegreeofNT4/5aminoacidsequencehomology.Therefore,thisELISAkitcanbeusedtoquantifyNT4/5inmanyspeciesincludingmouse,ratandmonkey.

TheantibodiesusedinthiskitbindtoepitopeswithinthematuredomainofNT4/5.Thus,thisELISAkitwillquantifythematureformofNT4/5,andmayalsodetectthefull-lengthpro-formofNT4/5.

ThisELISAkithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedfordiagnosticorclinicalprocedures.
RelatedproductsBL-003-1000,Heterophilicantibodyblocker:SamplediluentadditiveforaccuratequantificationorNT4/5inhumancitrate-plasma.
BatchNo.Refertotheproductlabel.
AntigenFUNCTION:Target-derivedsurvivalfactorforperipheralsensorysympatheticneurons.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Highestlevelsinprostate,lowerlevelsinthymus,placenta,andskeletalmuscle.Expressedinembryonicandadulttissues.SIMILARITY:BelongstotheNGF-betafamily.
OtherNamesNeurotrophin-5;NT-5;Neutrophicfactor5;Neurotrophin-4;NT-4;Neutrophicfactor4;NTF5;NTF4
AccessionNTF4_HUMAN
SpecificityHuman.
Cross-reactivityThecaptureanddetectionantibodieswillalsodetectNT4/5fromotherspeciesduetoahighdegreeofNT4/5aminoacidsequencehomology.Therefore,thisELISAkitcanbeusedtoquantifyNT4/5inmanyspeciesincludingmouse,ratandmonkey.
StorageStoreat2-8°C.
ExpiryDate12monthsfrompurchase.
SpecificReferencesMaejimaH,KanemuraN,KokubunT,MurataK,TakayanagiK(2017)Exerciseenhancescognitivefunctionandneurotrophinexpressioninthehippocampusaccompaniedbychangesinepigeneticprogramminginsenescence-acceleratedmice.NeurosciLett.doi:10.1016/j.neulet.2017.11.023.[Epubaheadofprint].Application:Mousehippocampushomogenates.

TakahashiK,MaejimaH,IkutaG,ManiH,AsakaT(2016)Exercisecombinedwithlow-levelGABAAreceptorinhibitionup-regulatestheexpressionofneurotrophinsinthemotorcortex.NeurosciLett.doi:10.1016/j.neulet.2016.10.052.[Epubaheadofprint].Application:Mousecortexhomogenates,innativecelllysisbuffer.
GeneralReferencesLodouichi(2000)J.Neuroscie.20(6):2155
Zhangetal.(1999)JNeurosci.Meths.89(1):69
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols.
Range15.6-1000pg/mL
SensitivityThisELISAkittypicallydetectsdownto25pg/mLhumanNT4/5(definedas150%ofblankvalue).
KitprotocolClick to download the kit protocolBEK-2218_as_at_March2017_Final.pdf
MSDSClick to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf