Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | TheBiosensisNeurotrophin4/5RapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthespecific,fastandreliablequantificationofNT4/5inlessthan4hoursincellculturesupernatants,humancitrate-plasmaandbrainextractsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularbloodsamples.AccuratequantificationofNT4/5inhumancitrate-plasmarequiresaheterophilicantibody(HA)blockerwhichcanbepurchasedseparately(BL-003-1000). | ThisELISAkitconsistsofapre-coatedpolyclonalanti-NT4/5captureantibody,abiotinylatedanti-NT4/5detectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproduct,whichisdirectlyproportionaltotheconcentrationofNT4/5presentinsamplesandproteinstandards. ThisNT4/5ELISAkitemploysarecombinanthumanNT4/5standard.ThecaptureanddetectionantibodieswillalsodetectNT4/5fromotherspeciesduetoahighdegreeofNT4/5aminoacidsequencehomology.Therefore,thisELISAkitcanbeusedtoquantifyNT4/5inmanyspeciesincludingmouse,ratandmonkey. TheantibodiesusedinthiskitbindtoepitopeswithinthematuredomainofNT4/5.Thus,thisELISAkitwillquantifythematureformofNT4/5,andmayalsodetectthefull-lengthpro-formofNT4/5. ThisELISAkithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedfordiagnosticorclinicalprocedures. Relatedproducts | BL-003-1000,Heterophilicantibodyblocker:SamplediluentadditiveforaccuratequantificationorNT4/5inhumancitrate-plasma. | BatchNo. | Refertotheproductlabel. | Antigen | FUNCTION:Target-derivedsurvivalfactorforperipheralsensorysympatheticneurons.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Highestlevelsinprostate,lowerlevelsinthymus,placenta,andskeletalmuscle.Expressedinembryonicandadulttissues.SIMILARITY:BelongstotheNGF-betafamily. | OtherNames | Neurotrophin-5;NT-5;Neutrophicfactor5;Neurotrophin-4;NT-4;Neutrophicfactor4;NTF5;NTF4 | Accession | NTF4_HUMAN | Specificity | Human. | Cross-reactivity | ThecaptureanddetectionantibodieswillalsodetectNT4/5fromotherspeciesduetoahighdegreeofNT4/5aminoacidsequencehomology.Therefore,thisELISAkitcanbeusedtoquantifyNT4/5inmanyspeciesincludingmouse,ratandmonkey. | Storage | Storeat2-8°C. | ExpiryDate | 12monthsfrompurchase. | SpecificReferences | MaejimaH,KanemuraN,KokubunT,MurataK,TakayanagiK(2017)Exerciseenhancescognitivefunctionandneurotrophinexpressioninthehippocampusaccompaniedbychangesinepigeneticprogramminginsenescence-acceleratedmice.NeurosciLett.doi:10.1016/j.neulet.2017.11.023.[Epubaheadofprint].Application:Mousehippocampushomogenates. | TakahashiK,MaejimaH,IkutaG,ManiH,AsakaT(2016)Exercisecombinedwithlow-levelGABAAreceptorinhibitionup-regulatestheexpressionofneurotrophinsinthemotorcortex.NeurosciLett.doi:10.1016/j.neulet.2016.10.052.[Epubaheadofprint].Application:Mousecortexhomogenates,innativecelllysisbuffer. GeneralReferences | Lodouichi(2000)J.Neuroscie.20(6):2155 | Zhangetal.(1999)JNeurosci.Meths.89(1):69 Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols. | Range | 15.6-1000pg/mL | Sensitivity | ThisELISAkittypicallydetectsdownto25pg/mLhumanNT4/5(definedas150%ofblankvalue). | Kitprotocol | BEK-2218_as_at_March2017_Final.pdf | MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf | 品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。
厂家直采
全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关,提供发票
及时交付
限时必达 不达必赔
|