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主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1
产品编号:BEK-2110-1P
市  场 价:¥8340.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$417.00
品      牌: Biosensis
公      司:Biosensis
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Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1
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Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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DescriptionThemouseVascularendothelialgrowthfactor(VEGF)KitisasandwichELISA.ThecaptureantibodyisaVEGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownVEGFconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundVEGFantibody.Abiotinylatedanti-mouseVEGFantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofVEGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseVEGFinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.

Vascularendothelialgrowthfactor(VEGF)isadisulfidelinkedhomodimericproteinassociatedwithangiogenesis,vasculogenesisandendothelialcellgrowth.Atleast10isoformsofVEGFareproducedbyalternativesplicingwithvaryinglevelsofexpression.TheisoformsVEGF188,VEGF164andVEGF120arewidelyexpressedwhereastheisoformsVEGF205andVEGF144areuncommon.IsoformsVEGF164andVEGF120arefreelysecretedwhereasisoformVEGF188remainscellassociated.
BatchNo.Seeproductlabels
AntigenThestandardandimmunogenusedintheELISAisrecombinantmouseVEGF-Aaminoacids27-190producedinandpurifiedfromaSF21insectcellexpressionsystem.
OtherNamesVEGF-A;VascularpermeABIlityfactor;VPF;VEGFA;VEGF;VEGF-1(VEGF164);VEGF-2(VEGF120)
AccessionQ00731VEGFA_MOUSE;
SpecificityMouseVEGF.Thiskitrecognizesboththe164and120aminoacidresidueformsofmouseVEGF.
StorageStoreat4°C
SpecificReferencesHuttonCP,DéryN,RosaE,LemonJA,RolloCD,BorehamDR,FahnestockM,deCatanzaroD,WojtowiczJM,BeckerS(2015)Synergisticeffectsofdietandexerciseonhippocampalfunctioninchronicallystressedmice.Neuroscience308:180-93.doi:10.10166.
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range15.6pg/ml-1,000pg/ml
Sensitivity< 2 pg/ml
KitprotocolClick to download the kit protocolBEK-2110-1Plate_as_at_Nov2015protocol.pdf