Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | ThemouseVascularendothelialgrowthfactor(VEGF)KitisasandwichELISA.ThecaptureantibodyisaVEGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownVEGFconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundVEGFantibody.Abiotinylatedanti-mouseVEGFantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofVEGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseVEGFinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. | Vascularendothelialgrowthfactor(VEGF)isadisulfidelinkedhomodimericproteinassociatedwithangiogenesis,vasculogenesisandendothelialcellgrowth.Atleast10isoformsofVEGFareproducedbyalternativesplicingwithvaryinglevelsofexpression.TheisoformsVEGF188,VEGF164andVEGF120arewidelyexpressedwhereastheisoformsVEGF205andVEGF144areuncommon.IsoformsVEGF164andVEGF120arefreelysecretedwhereasisoformVEGF188remainscellassociated. BatchNo. | Seeproductlabels | Antigen | ThestandardandimmunogenusedintheELISAisrecombinantmouseVEGF-Aaminoacids27-190producedinandpurifiedfromaSF21insectcellexpressionsystem. | OtherNames | VEGF-A;VascularpermeABIlityfactor;VPF;VEGFA;VEGF;VEGF-1(VEGF164);VEGF-2(VEGF120) | Accession | Q00731VEGFA_MOUSE; | Specificity | MouseVEGF.Thiskitrecognizesboththe164and120aminoacidresidueformsofmouseVEGF. | Storage | Storeat4°C | SpecificReferences | HuttonCP,DéryN,RosaE,LemonJA,RolloCD,BorehamDR,FahnestockM,deCatanzaroD,WojtowiczJM,BeckerS(2015)Synergisticeffectsofdietandexerciseonhippocampalfunctioninchronicallystressedmice.Neuroscience308:180-93.doi:10.10166. | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 15.6pg/ml-1,000pg/ml | Sensitivity | < 2 pg/ml | Kitprotocol | BEK-2110-1Plate_as_at_Nov2015protocol.pdf | 品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。
厂家直采
全球直采 正品优价
正品保障
厂家直发 有线跟踪
正规清关
CIF100%正规报关,提供发票
及时交付
限时必达 不达必赔
|