Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | ThemouseCathepsinBKitisasandwichELISA.ThecaptureantibodyisapolyclonalmouseCathepsinBantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownCathepsinconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundCathepsinantibody.AbiotinylatedmouseCathepsinBantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofCathepsinpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseCathepsininsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.ThecelllineusedtomakeCathepsinBstandardinthemouseCathepsinkitisNSOcells;theyaremurineB-cellplasmacytomaline. | BatchNo. | Seeproductlabels | OtherNames | CathepsinB1;Ctsb; | Accession | P10605CATB_MOUSE; | Storage | Storeat4øC | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. | Range | 156pg/ml-10,000pg/ml | Sensitivity | < 5 pg/ml | Kitprotocol | Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit. | 品牌介绍
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