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Description | The human Osteoprotegerin (OPG) Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-human OPG antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known OPG concentration are added to these wells and allowed to complex with the bound OPG antibody. A biotinylated human OPG polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPG present in the samples. The purpose of this kit is the in-vitro quantitative determination of human OPG in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
Batch No. | See product labels |
Other Names | Osteoclastogenesis inhibitory factor; Tumor necrosis factor receptor superfamily member 11B; |
Accession | O00300 TR11B_HUMAN; |
Specificity | Human OPG |
Storage | Store at 4øC |
Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
Range | 93.8 pg/ml - 6,000 pg/ml |
Sensitivity | < 5 pg/ml |
Kit protocol | Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。