Description | Detergentstypicallypresentincelllysisbuffersarethoughttodisruptorganellesandcompartmentsandincreasetheexposureofsolublephospho-proteinstophosphatasesandproteasestherebyresultinginuncontrolleddephosphorylationandproteolysis. Phospho-SureRTDNeuronalextractionbufferisoptimizedfortheextractionofphosphorylatedproteinsfromneuronalandothersofttissuestypes.Thebufferextractsthephosphoproteinsinanativestatewithouttheuseofharshdetergentsoroxidizers,anditisspeciallyformulatedtohelpmaintainphosphoproteinsandprotectthemfromdegradationbetterthantrADItionaldetergentbasedextractionbuffers. |
BatchNo. | Seeproductlabel |
Unitsize | 100mL |
OtherNames | Phosphosure |
Applications | PleasedownloadtheprotocolbelowfordetailedinstructionsonhowtousePhospho-Sureinneuronalandothersofttissues. |
FeaturedArticles | RefertothepublicationbySunejaetal(2006)wherethisbufferwasshowntoimproverecoveryofphospho-proteinssuchasERK1/2-PandSAP/JNK-Pinthedissectionandhomogenizationofguineapigbraintissue. |
Comments | Phospho-SureRTDisdesignedasbothanisolationandextractionbufferandforbestresultstheintacttissueshouldbebathedinice-coldPhospho-surebufferduringexcisionofthetissue.OncethedesiredtissuepieceisisolatedinPhospho-sureRTDitmaybetransferredtoasmallvolumeofice-coldPhospho-SureRTDfordisruption. |
Form | Powdercontainingnopreservatives.PreparationofPhospho-SureRTDisverysimple.SimplyaddsterileddH2Odirectlytothepowderedsolutioninthesuppliedbottle,swirlbrieflytodissolvethechemicalsanduse.Thematerialcontainsnopreservativessosteriletechniqueshouldalwaysbeused. |
Appearance | Whitepowder |
Reconstitution | Add93mlofsteriledoubledistilledwaterandmixbygentlyswirling,finalvolume100mlat20°C. |
Storage | Thedry,unopenedcontainershouldbestoredatroomtemperatureinadryordesiccatedlocationprotectedfromlight.Donotstoreintherefrigeratorunlessmaterialisinadry,moisturefreeenvironment.Materialishydroscopicsooncethesealisbrokenitshouldbehydratedandnotresealedwhiledry.Oncehydrated,thebuffercanbestoredat4°Cforupto3months.Solutioncanbefrozenbutclumpingmayoccuruponthawingandisnotrecommended. |
ExpiryDate | Powderedformatcanbestoredupto12monthsafterpurchase. |
SpecificReferences | 1.SunejaSK,MoZ,PotashnerSJ.(2006)Phospho-CREBandotherphospho-proteins:improvedrecoveryfrombraintissue.JNeurosciMethods.2006Jan30;150(2):238-41. 2.ElviraMass,DagmarWachten,AnnaC.Aschenbrenner,AndréVoelzmann,MichaelHochemail(2014)MurineCreld1ControlsCardiacDevelopmentthroughActivationofCalcineurin/NFATc1Signaling,DevelopmentalCellVolume28,Issue6,p711726 |
Bufferprotocol | B-100-PS_as_at_March2013.pdf |
MSDS | MSDS_B-100_B-200_as_at_Apr2014.pdf |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。