Description | Detergentstypicallypresentincelllysisbuffersarethoughttodisruptorganellesandcompartmentsandincreasetheexposureofsolublephospho-proteinstophosphatasesandproteasestherebyresultinginuncontrolleddephosphorylationandproteolysis. Phospho-SureRTDNeuronalextractionbufferisoptimizedfortheextractionofphosphorylatedproteinsfromneuronalandothersofttissuestypes.Thebufferextractsthephosphoproteinsinanativestatewithouttheuseofharshdetergentsoroxidizers,anditisspeciallyformulatedtohelpmaintainphosphoproteinsandprotectthemfromdegradationbetterthantrADItionaldetergentbasedextractionbuffers. |
BatchNo. | Seeproductlabel |
Unitsize | 200mL |
OtherNames | Phosphosure |
Applications | PleasedownloadtheprotocolbelowfordetailedinstructionsonhowtousePhospho-Sureinneuronalandothersofttissues. |
FeaturedArticles | RefertothepublicationbySunejaetal(2006)wherethisbufferwasshowntoimproverecoveryofphospho-proteinssuchasERK1/2-PandSAP/JNK-Pinthedissectionandhomogenizationofguineapigbraintissue. |
Comments | Phospho-SureRTDisdesignedasbothanisolationandextractionbufferandforbestresultstheintacttissueshouldbebathedinice-coldPhospho-sureRTDbufferduringexcisionofthetissue.OncethedesiredtissuepieceisisolatedinPhospho-sureRTDitmaybetransferredtoasmallvolumeofice-coldPhospho-SureRTDfordisruption. |
Form | Powdercontainingnopreservatives.PreparationofPhospho-SureRTDisverysimple.SimplyaddsterileddH2Odirectlytothepowderedsolutioninthesuppliedbottle,swirlbrieflytodissolvethechemicalsanduse.Thematerialcontainsnopreservativessosteriletechniqueshouldalwaysbeused. |
Appearance | Whitepowder |
Reconstitution | Add186mlofsteriledoubledistilledwaterandmixbygentlyswirling,finalvolume200mlat20°C. |
Storage | Thedry,unopenedcontainershouldbestoredatroomtemperatureinadryordesiccatedlocationprotectedfromlight.Donotstoreintherefrigeratorunlessmaterialisinadry,moisturefreeenvironment.Materialishydroscopicsooncethesealisbrokenitshouldbehydratedandnotresealedwhiledry.Oncehydrated,thebuffercanbestoredat4°Cforupto3months.Solutioncanbefrozenbutclumpingmayoccuruponthawingandisnotrecommended. |
ExpiryDate | Powderedformatcanbestoredupto12monthsafterpurchaseundercool,dryconditions. |
SpecificReferences | 1.SunejaSK,MoZ,PotashnerSJ.(2006)Phospho-CREBandotherphospho-proteins:improvedrecoveryfrombraintissue.JNeurosciMethods.2006Jan30;150(2):238-41. 2.ElviraMass,DagmarWachten,AnnaC.Aschenbrenner,AndréVoelzmann,MichaelHochemail(2014)MurineCreld1ControlsCardiacDevelopmentthroughActivationofCalcineurin/NFATc1Signaling,DevelopmentalCellVolume28,Issue6,p711726 |
Bufferprotocol | B-200-PS_as_at_March2013.pdf |
MSDS | MSDS_B-100_B-200_as_at_Apr2014.pdf |
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