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主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer/B-200-PS/200 mL
产品编号:B-200-PS
市  场 价:¥2400.00
场      地:美国(厂家直采)
产品分类: 生化试剂>其他>其他试剂>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$120.00
品      牌: Biosensis
公      司:Biosensis
公司分类:
Biosensis/Phospho-Sure RTD Neuronal Cell & Soft tissue extraction buffer/B-200-PS/200 mL
商品介绍
DescriptionDetergentstypicallypresentincelllysisbuffersarethoughttodisruptorganellesandcompartmentsandincreasetheexposureofsolublephospho-proteinstophosphatasesandproteasestherebyresultinginuncontrolleddephosphorylationandproteolysis.

Phospho-Sure™RTDNeuronalextractionbufferisoptimizedfortheextractionofphosphorylatedproteinsfromneuronalandothersofttissuestypes.Thebufferextractsthephosphoproteinsinanativestatewithouttheuseofharshdetergentsoroxidizers,anditisspeciallyformulatedtohelpmaintainphosphoproteinsandprotectthemfromdegradationbetterthantrADItionaldetergentbasedextractionbuffers.
BatchNo.Seeproductlabel
Unitsize200mL
OtherNamesPhosphosure
ApplicationsPleasedownloadtheprotocolbelowfordetailedinstructionsonhowtousePhospho-Sureinneuronalandothersofttissues.
FeaturedArticlesRefertothepublicationbySunejaetal(2006)wherethisbufferwasshowntoimproverecoveryofphospho-proteinssuchasERK1/2-PandSAP/JNK-Pinthedissectionandhomogenizationofguineapigbraintissue.
CommentsPhospho-Sure™RTD™isdesignedasbothanisolationandextractionbufferandforbestresultstheintacttissueshouldbebathedinice-coldPhospho-sure™RTD™bufferduringexcisionofthetissue.OncethedesiredtissuepieceisisolatedinPhospho-sure™RTD™itmaybetransferredtoasmallvolumeofice-coldPhospho-Sure™RTD™fordisruption.
FormPowdercontainingnopreservatives.PreparationofPhospho-­Sure™RTD™isverysimple.SimplyaddsterileddH2Odirectlytothepowderedsolutioninthesuppliedbottle,swirlbrieflytodissolvethechemicalsanduse.Thematerialcontainsnopreservativessosteriletechniqueshouldalwaysbeused.
AppearanceWhitepowder
ReconstitutionAdd186mlofsteriledoubledistilledwaterandmixbygentlyswirling,finalvolume200mlat20°C.
StorageThedry,unopenedcontainershouldbestoredatroomtemperatureinadryordesiccatedlocationprotectedfromlight.Donotstoreintherefrigeratorunlessmaterialisinadry,moisturefreeenvironment.Materialishydroscopicsooncethesealisbrokenitshouldbehydratedandnotresealedwhiledry.Oncehydrated,thebuffercanbestoredat4°Cforupto3months.Solutioncanbefrozenbutclumpingmayoccuruponthawingandisnotrecommended.
ExpiryDatePowderedformatcanbestoredupto12monthsafterpurchaseundercool,dryconditions.
SpecificReferences1.SunejaSK,MoZ,PotashnerSJ.(2006)Phospho-CREBandotherphospho-proteins:improvedrecoveryfrombraintissue.JNeurosciMethods.2006Jan30;150(2):238-41.
2.ElviraMass,DagmarWachten,AnnaC.Aschenbrenner,AndréVoelzmann,MichaelHochemail(2014)MurineCreld1ControlsCardiacDevelopmentthroughActivationofCalcineurin/NFATc1Signaling,DevelopmentalCellVolume28,Issue6,p711–726
BufferprotocolClick to download the protocolB-200-PS_as_at_March2013.pdf
MSDSClick to download the Material Safety Data SheetMSDS_B-100_B-200_as_at_Apr2014.pdf
品牌介绍

Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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