Description | mCherryisanengineeredderivativeofoneofafamilyofproteinsoriginallyisolatedfromCnidarians(jellyfish,seaanemonesandcorals).ThemCherryproteinwasderivedfromDsRed,aredfluorescentproteinfromso-calleddisccoralsofthegenusDiscosoma.DsRedisa223aminoacid~28kDaproteinsimilarinsizeandpropertiestoGFP,but,obviously,producesaredratherthanagreenFluorochrome.TheoriginalDsRedwasengineeredextensivelyintheTsienlabtopreventitfromformingtetramersanddimersandtomodifyandimprovethespectralproperties(1-3).TheresultingmonomericproteinisusefulforapplicationssuchasFörsterResonanceEnergyTransfer(FRET,alsoknownasFluorescenceResonanceEnergyTransfer).Severalfurthercyclesofmutation,directedmodificationandevolutionaryselectionproducedmCherry,whichismonomericandhasanexcitationmaximumat587nmandandemissionmaximumat610nm(4). |
Unitsize | 100µL |
Antigen | RecombinantfulllengthmCherry. |
AntibodyType | Polyclonal |
Isotype | IgY |
Producedin | Chicken |
Purity | AntibodyissuppliedasanaliquotofconcentratedIgYpreparation. |
Applications | WesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:2,000-1:5,000isCrecommendedforWB.Adilutionof1:500-1,000isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Theantibodyreactswithabandat~28-30kDacorrespondingtointactfull-lengthmCherrybyWesternblotonHEK293cellstransfectedwithmCherryvector.Ithasalsobeenusedsuccessfullyforimmunocytochemistry. |
SpeciesAgainst | Speciesindependent. |
AntibodyAgainst | mCherry |
Form | Lyophilizedwith3%trehalose,withoutpreservatives. |
Reconstitution | Reconstitutewith100µLsterile-filtered,ultrapurewater.Centrifugebrieflytoremoveanyinsolublematerial. |
Storage | Storelyophilised,unopenedvialat2-8°Corlower.Afterreconstitution,preparealiquotsandstoreat-20°Cto-80°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase(unopenedvial). |
GeneralReferences | 1.BairdGS,ZachariasDA,TsienRY.Biochemistry,mutagenesis,andoligomerizationofDsRed,aredfluorescentproteinfromcoral.ProcNatlAcadSciUSA.97:11984-9(2000). 2.GrossLA,BairdGS,HoffmanRC,BaldridgeKK,TsienRY.ThestructureofthechromophorewithinDsRed,aredfluorescentproteinfromcoral.ProcNatlAcadSciUSA.97:11990-5(2000). 3.HeikalAA,HessST,BairdGS,TsienRY,WebbWW.Molecularspectroscopyanddynamicsofintrinsicallyfluorescentproteins:coralred(dsRed)andyellow(Citrine).ProcNatlAcadSciUSA.97:11996-2001(2000). 4.ShanerNC,CampbellRE,SteinbachPA,GiepmansBN,PalmerAE,TsienRY.Improvedmonomericred,orangeandyellowfluorescentproteinsderivedfromDiscosomasp.redfluorescentprotein.NatureBiotechnology22:1567-1572(2004). |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。