Description | The6XHistagisashortpeptidesequenceof6histidineresidues.Epitopessuchasthe6XHistagareoftenincludedwiththetargetDNAatthetimeofcloningtoproducefusionproteinscontainingthetagsequence.Thisallowsanti-epitopetagantibodiessuchasthisonetoserveasauniversaldetectionreagentforanyrecombinantproteincontainingthistag.Anti-epitopeantibodiesareausefulalternativetogeneratingantibodiestoidentifyaspecificrecombinantprotein.The6XHismotifisoftenusedasatagonrecombinantproteinstofacilitatepurificationwithimmobilizedmetal-affinitychromatography. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Theantiserumwasproducedbyimmunizingrabbitwithsynthesizedpeptidecontaining6Histidineresidues. |
Producedin | Rabbit |
Purity | Affinitypurified |
Applications | ELISA,WB,IP,IF,IHC.WesternBlottingatasuggestedconcentrationof1:500~1:1000.ELISAatasuggestedconcentrationof1:2500.IP5µg/sample.IF5µg/mL.IHC5µg/mL.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Comments | Ready-to-usereagentforin-vitrolaboratoryresearchuseonly. |
Specificity | His-taggedfusionproteins |
Form | Liquid.PBS,pH7.4with0.02%sodiumazide. |
Storage | Stablefor1yearat-20°Cfromthedateofshipment.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoopeningthecap.Aliquottoavoidrepeatedfreezingandthawing. |
ExpiryDate | 12months |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。