Description | The6XHistagisashortpeptidesequenceof6histidineresidues.Epitopessuchasthe6XHistagareoftenincludedwiththetargetDNAatthetimeofcloningtoproducefusionproteinscontainingthetagsequence.Thisallowsanti-epitopetagantibodiessuchasthisonetoserveasauniversaldetectionreagentforanyrecombinantproteincontainingthistag.Anti-epitopeantibodiesareausefulalternativetogeneratingantibodiestoidentifyaspecificrecombinantprotein.The6XHismotifisoftenusedasatagonrecombinantproteinstofacilitatepurificationwithimmobilizedmetal-affinitychromatography. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Syntheticpeptidecontaining6Histidineresidues. |
Isotype | IgG2b |
Producedin | Mouse.MonoclonalclonenumberHIS.H8 |
Applications | WesternBlotting(WB),Immunocytochemistry(IC)andImmunoprecipitation(IP).Suggestedstartingdilutionsareasfollows:WBatdilutionsof1:1000,ICandIPatdilutionsof1:200.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | His-taggedfusionproteins |
Form | Liquid.PBS,pH7.4with0.05%sodiumazide. |
Storage | Stablefor1yearat-20°Cunopened.Afteropeningwerecommendaliquotingandstoringat-20°Cforupto6months.Avoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoopeningthecap. |
ExpiryDate | 12monthsafterdateofreceipt,unopened. |
SpecificReferences | 1.DittmannK.etal(2010)"NuclearEGFRshuttlinginducedbyionizingrADIationisregulatedbyphosphorylationatresidueThr654"FEBSLett.2010Sep24;584(18):3878-3884. 2.KolevM.V.etal(2010)"UpregulatingCD59:anewstrategyforprotectionofneuronsfromcomplement-mediateddegenerationPharmacogenomicsJ.2010Feb;10(1):12-9. |
References | 1.PotalaS.andVermaR.S.(2010)"Targetingheadandnecksquamouscellcarcinomausinganovelfusiontoxin-diphtheriatoxin/HN-1."MolBiolRep.2010Aug1. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。