![Biosensis/Mouse monoclonal antibody to rat capsaicin receptor (VR1, TRPV1, 819-838), [Clone BS397]: IgG/M-1714-100/100 µg](images/biosensis/201712/M-1714-100_Merged.jpg)
产品编号：M-1714-100
市 场 价：¥5940.00
场 地：美国(厂家直采)
联系QQ：1570468124
电话号码：4000-520-616
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info@ebiomall.com
商品介绍
| Description | Thecapsaicinreceptor(VR1,TRPV1)isaligand-activatednon-selectivecalciumpermeantcationchannelinvolvedindetectionofnoxiouschemicalandthermalstimuli.Thereceptorseemstomediateprotoninfluxandmaybeinvolvedinintracellularacidosisinnociceptiveneurons.Itisinvolvedinmediationofinflammatorypainandhyperalgesia.Sensitizedbyaphosphatidylinositolsecondmessengersystemactivatedbyreceptortyrosinekinases,whichinvolvesPKCisozymesandPCL.Activationbyvanilloids,likecapsaicin,andtemperatureshigherthan42degreesCelsius,exhibitsatime-andCa2+-dependentoutwardrectification,followedbyalong-lastingrefractorystate.MildextracellularacidicpH(6.5)potentiateschannelactivationbynoxiousheatandvanilloids,whereasacidicconditions(pHlessthan6)directlyactivatethechannel.Canbeactivatedbyendogenouscompounds,including12-hydroperoxytetraenoicacidandbradykinin.ActsasionotropicendocannABInoidreceptorwithcentralneuromodulatoryeffects.Triggersaformoflong-termdepression(TRPV1-LTD)mediatedbytheendocannabinoidanandamineinthehippocampusandnucleusaccumbensbyaffectingAMPAreceptorsendocytosis(Ref:uniprot.org). |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(C-GSLKPEDAEVFKDSMVPGEK)asapartoftheC-terminalratVR1protein(aa:819-838)hasbeenusedastheimmunogen. |
| Sequence | C-GSLKPEDAEVFKDSMVPGEK;aa819-838ratVR1 |
| AntigenLocation | C-terminal |
| AntigenLength | 20aminoacids |
| AntibodyType | MousemonoclonalIgG |
| Isotype | IgG2b,k-lightchain |
| Clone | BS397 |
| OtherNames | VR1;TransientreceptorpotentialcationchannelsubfamilyVmember1;TrpV1;osm-9-likeTRPchannel1;OTRPC1;Vanilloidreceptor1;Capsaicinreceptor;VR-1 |
| Accession | Uniprot:O35433;TRPV1_RAT |
| Producedin | Mouse |
| MolecularWeight | Monomer90-100kDainmousebrainextracts;dimer180-200kDacanbeobservedundersomeconditions |
| Purity | ProteinGpurifiedmouseimmunoglobulin |
| Applications | FlowCytometry:2µg/10^6cells. Westernblotting:0.5-2µg/mL,SDS-PAGEonBis-Trisgel4-12%,5%beta-mercaptoethanol,primaryantibodyO/Nincubationin5%skimmilk/TBST.Secondaryisanti-mouse-HRP,1/6000dilution,2hatroomtemperature.BlotdevelopedonLI-COR®C-DiGit®lotScanner. IHC:FrozenorPEGembeddedtissuestested(PEGembedding,seeKlosenPetal(1993)JHistochemCytochem.41(3):455-63).Conditionstested:1-10µg/mLinPBS,48hours,followedbydetectionviadirectlyconjugatedfluorescentanti-mousesecondary.Antibodynotyettestedonparaffinembeddedsections.Otherimmunohistochemistrymethodsnotyettestedbutareexpectedtobereactive. ICC:4%formaldehydefixedcellstested;requirespermeabilizationstepasantigenepitopeisintracellular.Suggestedprimaryantibodyconcentration:1-2µg/mL. Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Antibodyisspecificforrat/mouseVR1proteininwesternsandimmunofluorescentimmunohistochemistryonmousePEGfixedDRGtissues.Pre-absorptionwithimmunogenobliteratespositivestaining.Crossreactivitywithothernon-VR1proteinsisminimal;crossreactivitywithVR1fromotherspeciesnotyettested. |
| SpeciesAgainst | Rat/mouse,otherspeciesnotyettested |
| AntibodyAgainst | RatVR1 |
| Cross-reactivity | ThisantibodycloneisknowntoreactwithratandmouseTrpV1.Itispredictedtoreactwithguineapigduetosequencehomology. |
| Form | LyophilizedfromPBS,pH7.4with3%trehalose. |
| Appearance | Drypowder |
| Reconstitution | Reconstitutein100µLofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservativesbutwillcontain3%trehaloseandbuffersalts. |
| Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstability.Antibodycontainsnopreservatives.Storageat4ºCwithanappropriateantibacterialagent.USESterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened. |
| GeneralReferences | PengH.Y.(2008)TRPV1mediatestheuterinecapsaicin-inducedNMDANR2B-dependentcross-organreflexsensitizationinanesthetizedrats.AmJPhysiolRenalPhysiol.Nov;295(5):F1324-35. |
品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。
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