Description | Intercellularadhesionmolecule1(ICAM1)isasingle-pass type1membraneglycoproteinthatbelongstotheICAMfamily.ICAM1istypicallyexpressedonendothelialcellsandcellsoftheimmunesystem.ICAM-1isalsoexpressedinalltypesofglialcellsincludingastrocytesandmicroglia. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | E.coli-derivedhumanICAM1recombinantprotein(Position:L214-P532).HumanICAM1shares52%and48%aminoacid(aa)sequenceidentitywithmouseandratICAM1,respectively. |
OtherNames | ICAM1;ICAM-1;Intercellularadhesionmolecule1;CD54; |
Accession | P05362ICAM1_HUMAN; |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanICAM1(precursor)hasapredictedlengthof532residuesandaMWof58kDa.Aconcentrationof0.5-1µg/mlisrecommendedtodetectICAM1inparaffinembeddedtissues.Heatmediatedantigenretrievalisrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
Cross-reactivity | Human |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgNaN3. |
Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
ExpiryDate | 12monthsafterpurchase |
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