Description | Saporinisaribosome-inactivatingprotein(RIP)oftypeI.ThismonomericRNAN-glycosidasepurifiedfromseedsoftheplantSaponariaofficinalisalsoknownasSoapwort,iscapableofspecificdepurinationofeukaryoticribosomesthusarrestingproteinsynthesis.NoligandhasbeenidentifiedinsaporinhenceitsinABIlitytotransversethecellmembrane.Duetoitstoxicityandstabilityofthestructure,saporinhasprovenextremelyusefulforconstructionofimmunotoxins.Theexpectedmolecularweightofthepurifiedsaporinis29.5kDa. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | Saporin,wholemolecule |
OtherNames | Saponariaofficinalis;Commonsoapwort |
Accession | RIP6_SAPOF |
Producedin | Rabbit |
Purity | Wholeserum |
Applications | IHC,Immunofluorescence,ELISA,WesternBlot.Adilutionof1:200to1:2000isrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Confirmedtoreactwithpurifiedsaporin. |
Cross-reactivity | Nocross-reactivitywithothermoleculeshasbeenreported. |
Form | Lyophilised |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstability,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
ExpiryDate | 12monthsafterpurchase |
References | 1.Stirpe,F.etal(1983)BiochemJ216,617-625 2.Tazzari,P.L.etal(1988)CancerImmunolImmunother.26,231-236 3.Strocchi,P.etal(1992)JImmunolMethods.155,57-63 4.Savino,C.etal(2000)FEBSLett.470,239-243 5.Barbieri,L.etal(2000)JBiochem(Tokyo).128,883-889 6.Savino,C.etal(1998)ActaCrystallogrDBiolCrystallogr.54,636-638 7.Santanche,S.etal(1997)BiochemBiophysResCommun.234,129-132 |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。