Description | FUNCTION:Target-derivedsurvivalfactorforperipheralsensorysympatheticneurons.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Highestlevelsinprostate,lowerlevelsinthymus,placenta,andskeletalmuscle.Expressedinembryonicandadulttissues.SIMILARITY:BelongstotheNGF-betafamily. |
BatchNo. | Seeproductlabel |
Unitsize | 500µg |
Antigen | RecombinanthumanNT4 |
OtherNames | Neurotrophin-5;NT-5;Neutrophicfactor5;Neurotrophin-4;NT-4;Neutrophicfactor4;NTF5;NTF4 |
Accession | NT5_HUMAN |
Producedin | Sheep |
Purity | ProteinGpurifiedIgG |
Applications | IHC,ELISA(1site),WesternBlot,inhibitionofBIOLOGicalactivityinvitro/invivo.Recommendedtobeusedataconcentrationof2-10µg/mlforimmunohistochemistry,ELISAandWesternblotandinhibitionofbiologicalactivityinvitro.Useneatforinvivostudiesat2-10µg/ml(ED50).NotethattheconcentrationofNT4isgenerallylowinmosttissuesnevertheless,neonataltestesofratcanbeusedasagoodpositivecontrol.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Lessthan1%cross-reactivityagainstNGF,recombinanthumanBDNFand5%toNT3hasbeenshownbydotblot. |
Cross-reactivity | KnowntoreactwithNT4fromratandhuman,mouseandmonkey. |
Blastit | IfyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteinsfollowthelinkinAccessionfield,selectthenthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
Form | Lyophilised |
Reconstitution | Reconstitutein500µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | FeronFetal(2008)Neurotrophinexpressionintheadultolfactoryepithelium.BrainRes.1196:13-21Application:IHC;Species:Rat |
GeneralReferences | 1.Lodouichi(2000)J.Neuroscie.20(6):2155 2.Zhangetal.(1999)JNeurosci.Meths.89(1):69 |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。