Description | FUNCTION:Seemstopromotesthesurvivalofvisceralandproprioceptivesensoryneurons.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Brainandperipheraltissues.SIMILARITY:BelongstotheNGF-betafamily. |
BatchNo. | Seeproductlabel |
Unitsize | 50µg |
Antigen | Asyntheticpeptide(YAEHKSHRGEY)aspartofhuman,mouseandratNT3proteinconjugatedtoBSAhasbeenusedastheimmunogen. |
OtherNames | Neurotrophin-3;Neurotrophicfactor;HDNF;Nervegrowthfactor2;NGF-2;Ntf3;Ntf-3 |
Accession | NT3_HUMAN NT3_MOUSE NT3_RAT |
Producedin | Sheep |
Purity | Affinitypurifiedantibody |
Applications | IHC,ELISA,WB,inhibitionofBIOLOGicalactivity.Aconcentrationof1-10µg/mlisrecommendedforIHC,ELISA,WBandinhibitionofbiologicalactivityinvitro;2-10µg/ml(ED50)forinvivouse.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Acrossreactivityoflessthan0.1%tomouseNGF,recombinanthumanBDNFandNT4/5hasbeenshownbydotblot. |
Cross-reactivity | Thisantiserumisknowntoreactwithrat,chickenandhumanNT3. |
Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedIDbetweentheimmunogenusedhereindifferentspeciesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
Form | Lyophilised |
Reconstitution | Reconstitutein50µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
ExpiryDate | 12monthsafterpurchase |
References | 1.Zouhetal.(1996)Mol.Neurobio.13(3):185-97 2.Rushetal.(1997)Clin.Exp.Pharmacol.24:549-55 3.Tafreshietal.(1998)Neurosci.83(2):373-80 |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。