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主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/Mouse nerve growth factor (2.5S) protein/PE-019-100/100 µg
产品编号:PE-019-100
市  场 价:¥5940.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$297.00
品      牌: Biosensis
公      司:Biosensis
公司分类:
Biosensis/Mouse nerve growth factor (2.5S) protein/PE-019-100/100 µg
商品介绍
DescriptionMouseNGF(2.5S)wasisolatedfrommousesuBMAxillaryglandsbymethodofMobleyetal(1976)andisaformofbeta-NGFthathasidenticalBIOLOGicalproperties.NGFisknowntoregulatethesurvivalanddevelopmentofcertainsympatheticandsensoryneurons.Itisadimerwith2identicalpolypeptidechainsanddimericmolecularweightofapproximately26,500Da.IsolationandpurificationofNGFfrommousesubmaxillaryglandsyieldspreparationsofNGF(2.5S)withidenticalbiologicalactivitybutwithcleavagesattheaminoterminus(withthelossof8aminoacids)and/oratthecarboxy-terminus(withthelossofarginine).Thesepreparationsarenamed2.5NGF(seereferencebelow).
BatchNo.Seeproductlabel
Unitsize100µg
OtherNamesmouseNGF;betaNGF
AccessionNGF_MOUSE
ProducedinMouse
MolecularWeightThehomodimerhasamolecularweightof~26KDaandthemonomer~13KDaasconfirmedbySDSPAGE.
PurityGreaterthan90%(asdeterminedbySDSelectrophoresis)
Biol.activityTestedbystimulationof8dayoldchickembryodorsalrootganglia.
FormLyophilisedfrom0.1MaceticacidunderdryN2withvacuum.Appearsasnear-whitepowder.Solutionsterilefilteredpriortodrying.
ReconstitutionReconstitutewiththeadditionof100µlsterilewaterorphosphatebufferedsalineanddivideintosingleusealiquots.Flashfreezealiquotsinadryiceethanolbathandstoreat-20Cto-70Cforbestresults.
StorageShortterm:-20°C.Avoidrepeatedfreezingandthawing.Longerterm-70C.Seeexpirationdateforshelf-lifeestimates,actualtimesmayvarydependinguponexperimentalconditionsandlaboratoryhandling.
ExpiryDate12monthsuponpurchasefordrymaterial.Hydratedmaterial,5daysat4C,30days-20C,60days-70CforhighestactivityAvoidrepeatedfreezingandthawing.Useinsulatedstoragecontainersforbestresults.
SpecificReferencesCarterDRetal(2016)GlutathionebiosynthesisisupregulatedattheinitiationofMYCN-drivenneuroblastomatumOrigenesis.MolOncol.2016Jun;10(6):866-78.

GoodhillGJetal(2015)Thedynamicsofgrowthconemorphology.BMCBiol.2015Feb;13(10).

CoulsonEJetal(2015)Neurotrophin-tyrosinekinasereceptorsignaling.UnitedStatesPatentApplication20150344535.

Gearing,DPetal(2013)Afullycaninisedanti-NGFmonoclonalantibodyforpainreliefindogs.BMCVetRes.9:226.

MatusicaDetal(2013)Anintracellulardomainfragmentofthep75neurotrophinreceptor(p75(NTR))enhancestropomyosinreceptorkinaseA(TrkA)receptorfunction.JBiolChem.2013Apr19;288(16):11144-54.

YuanJetal(2013)Optimalityandsaturationinaxonalchemotaxis.NeuralComput.2013Apr;25(4):833-53.

SykesAMetal(2012)Theeffectsoftransmembranesequenceanddimerizationoncleavageofthep75neurotrophinreceptorbyγ-secretase.JBiolChem.2012Dec21;287(52):43810-24.

E.M.Forbesetal(2012)CalciumandcAMPLevelsInteracttoDetermineAttractionversusRepulsioninAxonGuidance.Neuron.2012May10;74(3):490-503.
GeneralReferences
MobleyWC,SchenkerA,ShooterEM(1976)Biochemistry.15(25)pp.5543-52.
品牌介绍

Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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