Description | StemCellFactor(SCF)isahematopoieticgrowthfactorthatstimulatestheproliferationofmastcells. SCFalsomediatescell-celladhesionandactssynergisticallywithothercytokines.TheactionsofSCFareexertedbybindingtotheSCFReceptor(PE-1243-50)whichresultsinreceptordimerization.SCFisatypeImembraneglycoproteinwith5potentialN-linkedglycosylationsites.SolubleSCFisproducedbyproteolyticcleavageoftheextracellulardomain. |
BatchNo. | Seeproductlabel |
Unitsize | 5µg |
OtherNames | Kitligand;C-kitligand;Stemcellfactor;SCF;Mastcellgrowthfactor;MGF;KITLG;MGF;SCF; |
Accession | P21583SCF_HUMAN; |
Producedin | Human-ADNAsequenceencodingthesignalpeptideandthematureStemCellFactor(SCF)proteinofhumanSCFwasexpressedinmodifiedhuman293cells. |
MolecularWeight | SCFmigratesasabroadbandbetween20and42kDainSDS-PAGEduetopost-translationalmodifications.UnmodifiedSCFhasapredictedmolecularmassof21.0kDa.SCFseparatesintoanumberofisoformswithapIbetween3.0and7.0in2DPAGEduetopost-translationalmodifications.ThiscompareswiththeunmodifiedSCFwithapredictedpIof5.76. |
Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
Biol.activity | TheED50ofSCFistypically10-20ng/mlasmeasuredinacellproliferationassayusingthehumangrowthfactor-dependentM-07ecellline. |
Form | PurifiedSCFconsistsof0-50%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
Storage | Lyophilizedproductsshouldbestoredat2-8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
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