Description | Hepatocytegrowthfactor(HGF)isamemberoftheplasminogenrelatedgrowthfactorfamily.TheHGFproteinisadimerformedfromanalphaandbetasubunitlinkedbyadisulfidebond.BothsubunitsoriginatefromtheendoproteolyticcleavageofacommonprecursorwhichisBIOLOGicallyinactive.HGFisexpressedinKupffercellaandsinusoidalendothelialcellsintheliverandpancreas. |
BatchNo. | Seeproductlabel |
Unitsize | 5µg |
OtherNames | HGF;HPTA;Scatterfactor;SF;Hepatocytegrowthfactoralphachain;Hepatocytegrowthfactorbetachain;Hepatopoeitin-A; |
Accession | P14210HGF_HUMAN; |
Producedin | Human-ADNAsequenceencodingthecompletehumanHepatocyteGrowthFactorproteinisoform1(comprisingthesignalpeptide,alphaandbetachains)wasexpressedinmodifiedhuman293cells. |
MolecularWeight | Underreducingconditions,theHGFproteinmigratesasabroadbandbetween80and110kDainSDS-PAGEduetopost-translationalmodifications,inparticularglycosylation.TheunmodifiedHGFhasapredictedmonomericmolecularmassof79.7kDa.TheproteinseparatesintoanumberofisoformswithapIbetween6and9in2DPAGE.TheunmodifiedHGFproteinhasapredictedpIof8.2 |
Purity | >95%,asdeterminedbySDS-PAGEandvisualisedbyCoomassieBrilliantBlue |
Biol.activity | TheED50ofHGFistypically20-40ng/mlasmeasuredinacellproliferationassayusingthehumanHepG2cellline. |
Form | TheHGFformulationconsistsof0-30%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
Storage | Lyophilisedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,andlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
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