Description | Nervegrowthfactor(NGF)isimportantforthedevelopmentandmaintenanceofthesympatheticandsensorynervoussystems.Itstimulatesdivisionanddifferentiationofsympatheticandembryonicsensoryneurons. |
BatchNo. | Seeproductlabel |
Unitsize | 5µg |
OtherNames | Beta-nervegrowthfactor;beta-NGF;NGFB; |
Accession | P01138NGF_HUMAN; |
Producedin | Human-ADNAsequenceencodingthehumanbetaNGFproteinsequence(containingthesignalpeptide,pro-peptideandthematurebetaNGFsequence)wasexpressedinmodifiedhuman293cells.Theoreticalpeptidesequence:YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT |
MolecularWeight | TheNGFproteinmigratesatapproximately12-16kDainSDS-PAGE.Ithasapredictedmolecularmassof13.5kDa.TheproteinseparatesintoanumberofisoformswithapIbetween9and10in2DPAGEduetopost-translationalmodifications.TheunmodifiedproteinhasapredictedpIof9. |
Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
Biol.activity | TheED50ofbetaNGFistypically0.2-1.0ng/mlasmeasuredinacellproliferationassayusingthehumangrowthfactordependentTF-1cellline. |
Form | Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,andlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
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