商品介绍
| Description | TheproteinnamedTrkB(alsonamedNeurotrophictyrosinekinasereceptortype2(NTRK2),GP145-TrkBorTropomyosin-relatedkinaseBisareceptortyrosinekinaseinvolvedinthedevelopmentandthematurationofthecentralandtheperipheralnervoussystemsandisimportantintheregulationofneuronsurvival,proliferation,migration,differentiation,andsynapseformationandplasticity.TrkBmayalsoplayaroleinneutrophin-dependentcalciumsignalinginglialcellsandmediatecommunicationbetweenneuronsandglia. TrkBistheprimaryreceptorforBDNF(brain-derivedneurotrophicfactor.TrkBalsobindsNT4andNT3butlessefficiently.Uponligand-binding,thereceptorundergoeshomodimerization,autophosphorylationandactivation.TrkBactivationrecruits,phosphorylatesand/oractivatesseveraldownstreameffectorsincludingSHC1,FRS2,SH2B1,SH2B2andPLCG1thateachregulatedistinctoverlappingsignalingcascadeswithincells.ThroughSHC1,FRS2,SH2B1,SH2B2,theseactivatetheGRB2-Ras-MAPKcascadethatregulates,forinstance,neuronaldifferentiationincludingneuriteoutgrowth.ThesesameeffectorsalsocontroltheRas-PI3kinase-AKT1signalingcascadethatmainlyregulatesgrowthandsurvival.TrkB,viaactivationofPLCG1andthedownstreamproteinkinaseC-regulatedpathways,alsocontrolssynapticplasticity,andthusplaysaroleinlearningandmemorybyregulatingbothshorttermsynapticfunctionandlong-termpotentiation.PLCG1alsoleadstoNF-Kappa-Bactivationandthetranscriptionofgenesinvolvedincellsurvival.OnesuchconsequenceisthatPLCG1activationviaTrkBisabletosuppressanoikis,theapoptosisresultingfromlossofcell-matrixinteractions.(Reference:www.uniprot.org) |
| BatchNo. | Seeproductlabel |
| Unitsize | 50µg |
| Antigen | Asyntheticpeptide(SNDDDSA[pS]PLHHIS)correspondingtothesequencearoundSer477(AA470-483)inhumanTrkB.InrodentTrkB,theimmunogencorrespondstoAA471-483(pS478). |
| OtherNames | GP145-TrkB;Neurotrophictyrosinekinasereceptortype2;TrkBtyrosinekinase;Tropomyosin-relatedkinaseB |
| Accession | Q16620NTRK2_HUMAN; |
| Producedin | Rabbit |
| Purity | Affinitypurified,andabsorbed. |
| Applications | WesternBlotting(0.52µg/mL).Celllysatesormembranepreparationspreparedfromisolatedbrainorspinalcordtissuesarerecommended.HighlypurifiedBSAisrecommendedformembraneblocking,althoughthisantibodyhasbeenshowntoworkonmembranesblockedwithskimmilk. Immunohistochemistry(1-5µg/mL).AntibodyhasbeenshowntoworkonPFAfixed,frozensections.TBSispreferredforbufferpreparation. Otherapplicationshavenotbeentested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Human.AntibodyhasbeenshowntobespecificforTrkBphosphorylatedonserine478byphospho-peptideabsorptiondotblots,andoncelllysatesfromcelllinesinducedwithretinoicacidandBDNF.Antibodydetectsaclearbandinretinoicacid(RA)andBDNF-treatedNSC34celllysatesat140kDaonly,indicatingthatthephosphorylatedTrkBreceptorisbeingdetected.Additionalnon-specificbandsatlowermolecularweightareobservedinbothlysateswiththeantibodyandthesebandshavenotbeencharacterized. |
| Cross-reactivity | Humanandmouse,otherspeciesnotyettestedbutitispredictedtoreactwithratandchickenTrkB-pS478basedonaminoacidhomologyatthesite. |
| Form | LyophilizedfromPBScontaining3%trehalose. |
| Reconstitution | Reconstitutein50µLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Storageoflyophilisedantibodycanbeat-20°C.Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | MatusicaDetal.(2016),Inhibitionofmotorneurondeathinvitroandinvivobyap75neurotrophinreceptorintracellulardomainfragment.JCellSci.2016Feb1;129(3):517-30.doi:10.1242/jcs.173864.Epub2015Oct26.Species:Mouse;Application:WB,spinalcordlysates. |
| GeneralReferences | 1.LaiK-O.etal.(2012),NatureNeuroscience15,1506-1515,doi:10.1038/nn.3237.2.ZhaoL.etal.(2009),JournalofCellScience122,3123-3136,doi:10.1242/jcs.047712. |
品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。
公司介绍
产品分类
TR-200-BG
PE-1243-50
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PE-1246-5
TR-100-BG
S-1754-500
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Diabetes & Obesity
Intracellular Trafficking
PE-1239-5
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B-100-PS
Receptors & Channel Molecules
2 Plate ELISA kits
M-1763-100-FT
TR-200-BGT
PE-1239-2
PE-1242-5
M-1249-100
Neurotrophic Factor Receptors
Cell Cycle Regulators
S-1759-100
PE-1606-100
PE-1762-100
B-200-PS
PE-019-100
PE-1241-5
PE-003-100
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BL-003-1000
PE-1246-2
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PE-1253-100
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BL-005-500
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M-1728-100
DNA Damage & Repair
PE-001-100
PE-1608-1
TR-400-AG
PE-1243-25
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PE-1608-5
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PE-1242-2
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BL-002-1250
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BL-001-1250
Cancer
PE-019-500
Stem Cells
Junction Proteins
TR-300-AG
Apoptosis
PE-1252-100
Growth Factors
Development
Alzheimer\'s / Parkinson\'s
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