请使用支持JavaScript的浏览器!
主营:抗体类、生化类试剂、ELISA试剂盒、组织染色细胞可视化试剂、针对神经科学和细胞疾病研究产品和纯化蛋白等
℡ 4000-520-616
℡ 4000-520-616
Biosensis/Mouse monoclonal antibody to rat p75NTR [MC192]: IgG1/M-006-100/100 µg
产品编号:M-006-100
市  场 价:¥5940.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$297.00
品      牌: Biosensis
公      司:Biosensis
公司分类:
Biosensis/Mouse monoclonal antibody to rat p75NTR [MC192]: IgG1/M-006-100/100 µg
商品介绍
DescriptionMonoclonalantibodyMC192againsttheratlowaffinitynervegrowthfactorreceptor(p75NTR)isderivedfromthefusionofSp2/0-Ag14myelomacellswithmouseimmunesplenocytes.MC192monoclonalantibodywasoriginallygeneratedbyChandlersetal.p75NTRwasoriginallydiscoveredasalowaffinitynervegrowthfactorreceptor.Lateritwasfoundthatitwasthereceptorforallneurotrophins.Itmediatessignalsofneurotrophinsforneuronalsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Recently,ithasbeenrevealedthatp75NTRnotonlyactsasthereceptorforneurotrophinsbutalsothereceptorformanyotherpathologicalligandssuchasprions,rABIesvirusandamyloidbeta.p75NTRalsoactsasaco-receptorforNOGOwhichmediatesinhibitorysignalsofmyelinassociatedprotein.p75NTRishighlyexpressedinanumberofnon-neuronalandneuronalcellsincludingmotorneuronsduringdevelopmentandalsoindamagedneurons.MC192recognizestheextracellulardomainoftheneurotrophinreceptorp75NTRinrat.MC192antibodymaybeusedforimmunocytochemicallocalisationofratcellsexpressingp75NTR,ELISAandwesternblot.ThisantibodyhasalsobeenusedfortheconstructionoftheMC192-saporinimmunotoxinforspecificeliminationofneuronalpopulationsinbasalforebraincholinergicneuronstogenerateananimalmodelforAlzheimer"sdisease.UsingFlowCytometry,thisantibodyhasfrequentlybeenemployedforpanningtoisolatep75NTR-expressingratcells.MC192hasapotentialuseastheligandforgenedeliveryintop75NTR-expressingratcellsviaareceptor-mediatedmechanism.FUNCTION:LowaffinityreceptorwhichcanbindtoNGF,BDNF,NT-3,andNT-4.Canmediatecellsurvivalaswellascelldeathofneuralcells.SUBUNIT:Homodimer;disulfide-linked.Interactswithp75NTR-associatedcelldeathexecutor.InteractswithNGFRAP1/BEX3.InteractswithTRAF2,TRAF4,TRAF6,PTPN13andRANBP9.InteractsthroughTRAF6withSQSTM1whichbridgesNGFRtoNTRK1(Bysimilarity).InteractswithBEX1.SUBCELLULARLOCATION:Membrane;single-passtypeImembraneprotein.DOMAIN:DeathdomainisresponsIBLeforinteractionwithRANBP9.PTM:N-andO-glycosylated.PTM:Phosphorylatedonserineresidues.SIMILARITY:Contains1deathdomain.SIMILARITY:Contains4TNFR-Cysrepeats.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenNGFreceptor
AntigenLocationECDofratNGFR
IsotypeIgG1
CloneMC192
OtherNamesLow-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR
AccessionTNR16_RAT
ProducedinMouse
PurityProteinGpurifiedimmunoglobulin
ApplicationsIH(likelyfixed),ELISA,WB,FlowCytometry(2µgper10^6cells)IP(non-reducingconditionsonly!;donotusereducingagentssuchasDTTorbeta-mercaptoethanol),TraditionalformalinfixedparaffinembeddedimmunohistochemistryisNOTrecommendedwithMC192.Motorneuronisolation,Gene/ToxinDeliverytoratsensory/motorneurons.Aworkingsolutionof1-2µg/mlwasdeterminedbyimmunohistochemicalstainingon4%paraformaldehydefixed,oralcoholfixedratspinalcordandbrain.Fornon-denaturedWB,1-5ug/mLwasfoundtobesuitablewithsuitablecontrols(PC12lysate).ELISA:detectiononly,1-5ug/mLhasbeensuggestedinliterature.Immunoprecipitation:5μg/mL,>0.5%tritonX-100buffer/500ug/lysate;PC12positivecontrolstrongsuggested.MC192isnotsuitableasablockingagent,althoughithasbeenincorrectlyusedforthispurposeinmanypublishedworks.TheantibodywasgeneratedspecificallybyscreeningformonoclonalsthathadtheabilitytoENHANCEthebindingofNGF,thenaturalligandforp75.Therefore,thisantibodyisparticularlyunusual.Thefulldetailscanbefoundintheoriginalpaper,whichislistedonourdatasheet(seeChandleretal,1984).Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityMC192isspecificonlyforRATNGFR,noreactivitytoHumanorMouseNGFRhasbeenreported
Cross-reactivityThismonoclonalantibodyhasbeentestedforimmunohistochemicallocalisationofp75NTR-expressingratcellsinthespinalcordandbrain.Thismonoclonalantibodydoesnotcrossreactwithp75NTR-expressingcellsinotherspecies.
FormLyophilised
ReconstitutionReconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.
StorageTheMC192issuppliedinlyophilisedformfromProteinG-purifiedhybridomacellculturesupernatants.Thelyophilisedantibodyisstablewhenstoredat2-8°Cor-20°C.Afterreconstitutionundilutedaliquotsshouldbekeptat-20°Cforuptosixmonths.ForadditionalstabilityGlycerol(1:1)maybeaddedafterreconstitution.Repetitivefreeze/thawcycleshouldbeavoided.
SpecificReferencesRiffaultB,KourdougliN,DumonC,FerrandN,BuhlerE,SchallerF,ChambonC,RiveraC,GaiarsaJL,PorcherC(2016)Pro-Brain-DerivedNeurotrophicFactor(proBDNF)-Mediatedp75NTRActivationPromotesDepolarizingActionsofGABAandIncreasesSusceptibilitytoEpilepticSeizures.Cereb.Cortex[Epubaheadofprint].Application:WesternBlot;Species:Rat

BrandliA,JohnstoneDM,StoneJ(2016)RemoteIschemicPreconditioningProtectsRetinalPhotoreceptors:EvidenceFromaRatModelofLight-InducedPhotoreceptorDegeneration.InvestOphthalmolVisSci.57(13):5302-13Application:WesternBlot,IHC;Species:Rat

RiffaultB,MedinaI,DumonC,ThalmanC,FerrandN,FriedelP,GaiarsaJL,PorcherC.(2014)"Pro-Brain-DerivedNeurotrophicFactorInhibitsGABAergicNeurotransmissionbyActivatingEndocytosisandRepressionofGABAAReceptors."J.Neurosci.34(40):13516-34Application:WesternBlot,Neuronalcellsandhippocampi;Species:Rat

KalincikTetal(2011)SelectedchangesinspinalcordmorphologyafterT4transectionandolfactoryensheathingcelltransplantation.AutonNeurosci.158(1-2):31-8Application:IF;Species:Rat

WuAetal(2011)Delayedolfactoryensheathingcelltransplantsreducenociceptionafterdorsalrootinjury.ExpNeurol.229(1):143-57Application:IF;Species:Rat

DaviesAetal(2010)Thealpha2deltasubunitsofvoltage-gatedcalciumchannelsformGPI-anchoredproteins,aposttranslationalmodificationessentialforfunctionProcNatlAcadSciUSA.Jan26;107(4):1654-9

KalincikTetal(2010)Olfactoryensheathingcellsreducedurationofautonomicdysreflexiainratswithhighspinalcordinjury.AutonNeurosci.154(1-2):20-9Application:IHC;Species:Rat

Wilson-GerwingT.D.etal(2009)JCompNeurol.2009Sep1;516(1):49-58

FeronFetal(2008)Neurotrophinexpressionintheadultolfactoryepithelium.BrainRes.1196:13-21Application:IHC;Species:Rat

BiancoJIetal(2004)Neurotrophin3promotespurificationandproliferationofolfactoryensheathingcellsfromhumannose.Glia.45(2):111-23Application:IHC,IF;Species:Rat

EylesDetal(2003)Neuroscience.2003;118(3):641-53.Application:IHC;Species:Rat

LuJetal(2001)Transplantationofnasalolfactorytissuepromotespartialrecoveryinparaplegicadultrats.BrainRes.889(1-2):344-57Application:IF;Species:Rat
GeneralReferencesVilarM.etal(2009)Activationofthep75neurotrophinreceptorthroughconformationalrearrangementofdisulphide-linkedreceptordimersNeuron.2009Apr16;62(1):72-83
Luther,J.A.andBirren,S.J.(2009)J.Neurosci29,5411-24
Lagares,A.etal.(2007)J.Neurosci27,7939-53
Kruger,G.M.etal.(2002)Neuron35,657-69
Tuffereau,C.etal(1998)EmboJ17,7250-9
Zhou,X.F.,andRush,R.A.(1996)JCompNeurol372,37-48
Huber,J.,Dittrich,F.,andPhelan,P.(1993)EurJBiochem218,1031-1039
RabizadehS.etal(1993)Science.Jul16;261(5119):345-8.
Stemple,D.L.&Anderson,D.J.(1992)Cell71,973-85
Urschel,B.A.&Hulsebosch,C.E.(1992)BrainResDevBrainRes69,261-70
BirrenSJetal(1992)Science.Jul17;257(5068):395-7.
Yan,Q.,andJohnson,E.M.,Jr.(1988)JNeurosci.1988Sep;8(9):3481-98.
Johnson,D.etal.(1986)Cell47,545-54
Chandler,C.E.etal(1984)(1984)JBiolChem259,6882-6889
品牌介绍

Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔