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℡ 4000-520-616
℡ 4000-520-616
Biosensis/Mouse monoclonal antibody to rat p75NTR [MC192] - ATTO 488/M-026-50-AT/50 µg (1mg/ml)
产品编号:M-026-50-AT
市  场 价:¥5940.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
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美  元  价:$297.00
品      牌: Biosensis
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公司分类:
Biosensis/Mouse monoclonal antibody to rat p75NTR [MC192] - ATTO 488/M-026-50-AT/50 µg (1mg/ml)
商品介绍
DescriptionMonoclonalantibodyMC192againsttheratlowaffinitynervegrowthfactorreceptor(p75NTR)isderivedfromthefusionofSp2/0-Ag14myelomacellswithmouseimmunesplenocytes.MC192monoclonalantibodywasoriginallygeneratedbyChandlersetal.p75NTRwasoriginallydiscoveredasalowaffinitynervegrowthfactorreceptor.Lateritwasfoundthatitwasthereceptorforallneurotrophins.Itmediatessignalsofneurotrophinsforneuronalsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Recently,ithasbeenrevealedthatp75NTRnotonlyactsasthereceptorforneurotrophinsbutalsothereceptorformanyotherpathologicalligandssuchasprions,rABIesvirusandamyloidbeta.p75NTRalsoactsasaco-receptorforNOGOwhichmediatesinhibitorysignalsofmyelinassociatedprotein.p75NTRishighlyexpressedinanumberofnon-neuronalandneuronalcellsincludingmotorneuronsduringdevelopmentandalsoindamagedneurons.MC192hasapotentialuseastheligandforgenedeliveryintop75NTR-expressingratcellsviaareceptor-mediatedmechanism.FUNCTION:LowaffinityreceptorwhichcanbindtoNGF,BDNF,NT-3,andNT-4.Canmediatecellsurvivalaswellascelldeathofneuralcells.SUBUNIT:Homodimer;disulfide-linked.Interactswithp75NTR-associatedcelldeathexecutor.InteractswithNGFRAP1/BEX3.InteractswithTRAF2,TRAF4,TRAF6,PTPN13andRANBP9.InteractsthroughTRAF6withSQSTM1whichbridgesNGFRtoNTRK1(Bysimilarity).
BatchNo.Seeproductlabel
Unitsize50µg(1mg/ml)
AntigenRatp75NTR
IsotypeIgG1
CloneMC192
OtherNamesLow-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR
AccessionTNR16_RAT
ProducedinMouse
PurityImmunoglobulin(IgG1)waspurifiedusingProteinGcolumn(AmershamPharmacia),polishedwithSephacryl200HR(AmershamPharmacia)inPBS.TheIgGwasthenconjugatedtoATTO488(ATTOTEC)andpurifiedviagelfiltrationusingaG25finegraingelin10mMTris/50mMNaClsolution.
ApplicationsIF:liveorlightlyfixedcellsortissues(acetoneor4%PFA):2-5ug/mL.Notsuitableforwesternblots;notsuitableforIHonformalinfixedtissues.FACS(20ug/mL)isrecommended,unfixedcells.
SpecificityMC192recognizestheextracellulardomainoftheneurotrophinreceptorp75NTRinratanddoesnotreactwithhumanormouseNGFR.
SpeciesAgainstRAT
AntibodyAgainstn-octoglucosidestabilizedproteinscontainingp75receptor,whichwereisolatedfromplasmamembranesofPC-12cells(Chandleretal.,1984).
Cross-reactivityReactswithrat.DoesnotreactwithmouseorhumanNGFR
FormLiquid
StorageTheantibodyconjugatecanbestoredat4ºCforupto4monthswiththeadditionofappropriateantibacterialagent.
SpecificReferences1.DaviesA.etal(2010)Thealpha2deltasubunitsofvoltage-gatedcalciumchannelsformGPI-anchoredproteins,aposttranslationalmodificationessentialforfunctionProcNatlAcadSciUSA.Jan26;107(4):1654-9
References1.Chandler,C.E.etal(1984)JBiolChem259,6882-6889
2.LagaresAetal(2007)Jofneurosci27(30),7939-7953
品牌介绍

Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。

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