Description | TheRETproto-oncogeneisareceptortyrosinekinaseformembersoftheglialcellline-derivedneurotrophicfactorfamilyofextracellularsignallingmolecules |
Relatedproducts | M-918-100mousemonoclonalantibodytohumanRET[1C6]:IgG; |
BatchNo. | Seeproductlabel |
Antigen | RecombinantHis-taggedextracellularfragmentofhumanRETproteinproducedusingCHOcellline.TheextracellularfragmentofhRETwasexpressedandsecretedtothecellculturesupernatant.ProteinwaspurifiedbyNi-affinitychromatographyfollowinggel-filtrationfromcellculturesupernatant. |
AntibodyType | Polyclonal |
Isotype | IgY |
OtherNames | Proto-oncogenetyrosine-proteinkinasereceptorRet,Cadherinfamilymember12,Proto-oncogenec-Ret |
Producedin | Chicken |
Purity | affinitypurified |
Applications | WB,ICC.Adilutionof1:5000to1:10000isrecommendedforWesternblotand1:250to1:500forimmunocytochemistry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | HumanRET |
SpeciesAgainst | Human |
Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedIDbetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
Form | Liquid:ConcentratedammoniumsulphateinPBSpH7.4 |
Appearance | Clearliquid |
Reconstitution | Storeantibodystocksolutionat+4°Cuponreceipt.DONOTFREEZE.Thisproductisan(NH4)2SO4precipitate.Touse,mixwellbypipettingorvortexinggentlyprioruse,thenwithdrawthedesiredamountofantibodystocksolutionanddiluteintoyourantibodydilutionbufferandusenormally.Alternatively,materialcanbecentrifugedat10Kxgfor5minutesandtheliquidsulfatesolutioncarefullyremoved.ThenprecipitatedantibodycanberesUSPendedin0.1MP,250mMNaCL,pH7.4solution.Thesolutionshouldthenbedividedintoworkingaliquotsandstoredat-80Cuntilused.Preventmultiplefreezethawcyclesofaliquotsforbestproductlife. |
Storage | Storeat+4°Cuponreceipt;DONOTFREEZE.Asproductis(NH4)2SO4(ammoniumsulfate)precipitate,mixwellbypipettingorvortexingpriortouse.Seereconstitutioninstructionsformoreinformation. |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。