Description | TheGFRa-3isaglycosylphosphatidylinositol(GPI)-linkedcellsurfacereceptorandamemberoftheGDNFreceptorfamily.ItformsasignalingreceptorcomplexwithRETtyrosinekinasereceptorandbindstheligand,artemin. |
Relatedproducts | R-068-100RabbitantibodytoGFRalpha-3(1-11):wholeserum;C-1659-100ChickenantibodytoGFRalpha-1:affinitypurified;C-1660-100ChickenantibodytoGFRalpha-2:affinitypurified;C-1662-100chickenantibodytoGFRalpha-4:affinitypurified; |
BatchNo. | Seeproductlabel |
Antigen | RecombinantHis-taggedhumanGFRa-3proteinproducedusingCHO-basedcellline.ForproductionofhGFRa-3,glycosylphosphatidyl-inositolGPI-anchorwasremovedandproteinwassecretedtothecellculturesupernatant.ProteinwaspurifiedbyNi-affinitychromatographyfollowinggel-filtrationfromcellculturesupernatant. |
Sequence | UniprotO60609 |
AntibodyType | Polyclonal |
Isotype | IgY |
OtherNames | GDNFreceptoralpha-3 |
Accession | O60609 |
Producedin | Chicken |
Purity | Affinitypurified |
Applications | WB,ICC.Adilutionof1:5000to1:10000isrecommendedforWesternblotand1:1500to1:3000forimmunocytochemistry.Biosensisrecommendsthatoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | HumanGFRa-3.Nocross-reactivitywithhGFRa-1,hGFRa-2,hGFRa-4. |
SpeciesAgainst | Human |
Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedIDbetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
Form | ConcentratedammoniumsulphateinPBSpH7.4containnopreservatives |
Reconstitution | Storeantibodystocksolutionat+4°Cuponreceipt.DONOTFREEZE.Thisproductisan(NH4)2SO4precipitate.Touse,mixwellbypipettingorvortexinggentlyprioruse,thenwithdrawthedesiredamountofantibodystocksolutionanddiluteintoyourantibodydilutionbufferandusenormally. Alternatively,materialcanbecentrifugedat10Kxgfor5minutesandtheliquidsulfatesolutioncarefullyremoved.ThenprecipitatedantibodycanberesUSPendedin0.1MP,250mMNaCL,pH7.4solution.Thesolutionshouldthenbedividedintoworkingaliquotsandstoredat-80Cuntilused.Preventmultiplefreezethawcyclesofaliquotsforbestproductlife. |
Storage | Storeat+4°Cuponreceipt;DONOTFREEZE.Asproductis(NH4)2SO4(ammoniumsulfate)precipitate,mixwellbypipettingorvortexingpriortouse.Seereconstitutioninstructionsformoreinformation. |
ExpiryDate | 12monthsafterpurchase. |
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