Description | Endomorphins1and2areendogenousopioidpeptideswhichhavethehighestaffinityforthemu-opioidreceptors.Locatedinvariouspartsofthebrainandinteractswithmu-opioidreceptorsandproducesanalgesia. |
BatchNo. | Seeproductlabel |
Unitsize | 50µg |
Antigen | AntibodiestohumanEndomorphins1and2weremadebyimmunisingrabbitswiththehumanEndomorphin-1peptide(YPWF)andthenselectingthoseantibodiesthatcross-reactedwithbothhumanEndomorphins1and2. |
OtherNames | EM1;EM2; |
Producedin | Rabbit |
Applications | Adilutionof5-10µg/mlisrecommendedforimmunohistochemistryusingformalinfixedandparaffinembeddedtissuesandfor4%paraformaldehydefixedfrozentissues.Adilutionof5-15µg/mlisrecommendedforimmunofluorescence..Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
SpeciesAgainst | Human |
AntibodyAgainst | Endomorphin-1,Endomorphin-2 |
Cross-reactivity | Human;mouse;rat.Endomorphin-1ishighlyconservedmoleculesocross-reactivitywithotherspeciesisexpected.Cross-reactivitywithotheropioidpeptidesisasfollows:withEndomorphin-2=70%;Met-enkephalin<0.03%; with Leu-enkephalin 0.01%; with beta-endorphin<0.1%. |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenproteins.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
BlastURL | Clickhere |
Form | LyophilisedwithBSA |
Appearance | Whitepowder |
Reconstitution | Reconstitutein0.05mlofPBS(pH7.4)toachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。