Description | PAFAHalphaencodesthenon-catalyticalphasubunitoftheintracellularIbisoformofplatelet-activatingfactoracteylhydrolase,aheterotrimericenzymethatspecificallycatalyzestheremovaloftheacetylgroupattheSN-2positionofplatelet-activatingfactor.Twootherisoformsofintracellularplatelet-activatingfactoracetylhydrolaseexist:onecomposedofmultiplesubunits,theother,asinglesubunit.Inaddition,asingle-subunitisoformofthisenzymeisfoundinserum.Itisexpressedubiquitouslyinboththefrontalandoccipitalareasofthebrain.DefectsinPAFAHalphacauselissencephalytype1(LIS1)andofsubcorticalbandheterotopia(SBH).DefectsinPAFAHalphaarealsoacauseofMiller-Diekerlissencephalysyndrome(MLDS). |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | PartialrecombinanthumanPAFAHalpha(1-111)withaGSTtag. |
Clone | 2C12 |
OtherNames | Platelet-activatingfactoracetylhydrolaseIBsubunitalpha;PAFacetylhydrolase45kDasubunit;PAF-AH45kDasubunit;PAF-AHalpha;PAFAHalpha;Lissencephaly-1protein;LIS-1;PAFAH1B1;LIS1;MDCR;MDS;PAFAHA |
Accession | LIS1_HUMAN |
Producedin | Mouse |
Purity | ProteinGpurifiedimmunoglobulin |
Applications | ThisantibodyisrecommendedforWBandsandwichELISA.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | SpecificityhasbeenconfirmedbyWBanddirectELISAagainsttheantigen. |
Cross-reactivity | Human.Otherspecieshavenotbeentested. |
Blastit | Toseethesharedidentitybetweendifferentspeciesorotherproteins,followthelinkintheAccessionfield,selectthesequencethatyouareinterestedinandcopyandpasteitHEREandblast/formatit. |
Form | LyophilisedfromPBSpH7.2 |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20ºCforhigherstABIlityorat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforadditionalstability.Avoidrepetitivefreeze/thawcycles. |
ExpiryDate | 12monthsafterpurchase |
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