Description | Talinisalarge,multidomainfocaladhesionproteininvolvedintheassemblyofactinfilaments.Talininteractswiththeintracellulardomainsofintegrins,vinculinandotherfocaladhesionproteins. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Talinpurifiedfromchickengizzard |
Clone | Tal-33 |
OtherNames | Talin-1;TLN1;KIAA1027;TLN;Talin-2;TLN2;KIAA0320; |
Accession | P54939TLN1_CHICK;Q9Y490TLN1_HUMAN;Q9Y4G6TLN2_HUMAN; |
Producedin | Mouse |
Purity | IgG |
Applications | WesternBlotting(WB).Aconcentrationof2.0-4.0µg/mlisrecommendedtodetecttheproteinincellsandtissues.ThisantibodyreactswithbothTalin-1andTalin-2.ItrecognizesanepitopelocatedontheintactTalinanda190kDafragment.Itdoesnotrecognizethe47kDafragmentobtainedbyproteasecleavageofchickengizzardTalin.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
Cross-reactivity | Human;mouse;rat;chicken;frog; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilizedfrom1.2%sodiumacetate,2mgBSA,0.01mgNaN3 |
Reconstitution | Reconstitutein1mlofPBS(pH7.4)toachieveanantibodyconcentrationof100µg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。