Description | Neurofilamentscanbedefinedastheintermediateor10nmdiameterfilamentsfoundinneuronalcells.Theyarecomposedamixtureofsubunitswhichoftenincludestheneurofilamenttripletproteins,NF-L,NF-MandNF-H.Neurofilamentsmayalsoincludeperipherin,alpha-internexin,nestinandinsomecasesvimentin.Alpha-internexinisa~66kDaClassIVintermediatefilamentsubunitexpressedinlargeamountsearlyinneuronaldevelopment,butisdownregulatedinmanyneuronsasdevelopmentprocedes.Manyclassesofmatureneuronscontainalpha-internexininadditiontoNF-L,NF-MandNF-H.Insomematureneuronsalpha-internexinistheonlyneurofilamentsubunitexpressed.Antibodiestoalpha-internexinarethereforeuniqueprobestostudyandclassifyneuronaltypesandfollowtheirprocessesinsectionsandintissueculture.Inadditiontheveryearlydevelopmentalexpressionofalpha-internexinmeansitspresenceisanearlyandconvenientdiagnosticfeatureofneuronalProgenitorscellsandothercellcommittedtotheneuronallineage. |
BatchNo. | Seeproductlabel |
Unitsize | 250µl |
Antigen | Recombinantratalpha-internexinexpressedandpurifiedfromE.coli |
AntibodyType | Monoclonal |
Isotype | IgG1 |
Clone | 1D2 |
OtherNames | 66kDaneurofilamentprotein;NF-66;Neurofilament-66;Alpha-internexin;Alpha-Inx;Neurofilament5;INA;NEF5; |
Accession | Q16352AINX_HUMAN;P23565AINX_RAT; |
Producedin | Mouse |
Applications | WesternBlotting(WB),Immunocytochemistry(IC)andImmunohistochemistryonparaffinsections(IHC).TherecommendeddilutionforWBis1:5000-10,000.TheepitoperecognisedbythisantibodyisintheC-terminalnon-helicalextensionoftheproteinandisunusuallyresistanttoaldehydefixationsothisantibodyisideallysuitedforstudiesofparaffinembeddedformalinfixedhistologicalsections.TherecommendeddilutionforIHCis1:1,000-5,000.ForIC,dilutionis1:250-500.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB.Thisantibodyisspecificforthe64-66kDaalpha-internexinprotein.Molecularweightwilldependonspecies. |
AntibodyAgainst | Internexinalpha |
Cross-reactivity | Hu,Rat,Ms,Fel,andothermammals |
BlastURL | Clickhere |
Form | Lyophilisedwith5%trehalose |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。