Description | Excitatoryaminoacidtransporter1(EAAT1)isaglutamatetransporter(GluT).ItrapidlyremovesL-glutamatefromthesynapticcleftwhichisessentialforterminatingthepostsynapticactionofglutamate.EAAT1isalsoknownashighaffinitysodium-dependentglutamate/aspartatetransporter1(GLAST1).EAAT1ishighlyexpressedincerebellumbutisalsofoundinthefrontalcortex,hippocampusandbasalganglia. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(ENEMKKPYQLIAQDNE)correspondingtoaregion516-531fromtheC-terminusofhumanEAAT1/GLAST1. |
OtherNames | SLC1A3;GLAST;GLT-1; |
Accession | P43003EAA1_HUMAN;P24942EAA1_RAT |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | WesternBlotting(WB).Aconcentrationof0.5-1.0µg/mlisrecommendedforWB.HumanEAAT1hasapredictedlengthof542residuesandaMWof60kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen. |
Cross-reactivity | Human;mouse;rat; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilised |
Reconstitution | Eachvialcontains5mgofBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosaland0.05mgNaN3.Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlityandat4°Cwithanappropriateantibacterialagent.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。