Description | Interleukin-1beta(IL-1β)alsoknowascatabolin,isacytokineproteinthatinhumansisencodedbytheIL1Bgene,whichlocalizestothelongarmofchromosome2.Interleukin1wasdiscoveredbyGeryin1972.Henameditlymphocyte-activatingfactor(LAF)becauseitwasalymphocytemitogen.Itwasnotuntil1985thatinterleukin1wasdiscoveredtoconsistoftwodistinctproteins,nowcalledinterleukin-1alphaandinterleukin-1beta.IL-1βisamemberoftheinterleukin1cytokinefamily.Thiscytokineisproducedbyactivatedmacrophagesasaproprotein,whichisproteolyticallyprocessedtoitsactiveformbycaspase1(CASP1/ICE).Thiscytokineisanimportantmediatoroftheinflammatoryresponse,andisinvolvedinavarietyofcellularactivities,includingcellproliferation,differentiation,andapoptosis.Theinductionofcyclooxygenase-2(PTGS2/COX2)bythiscytokineinthecentralnervoussystem(CNS)isfoundtocontributetoinflammatorypainhypersensitivity.Reference:http://en.wikipedia.org/wiki/IL1B BiosensisrabbitpolyclonaltomouseIL-1βhasbeentestedagainstmousetissuelysatesandreactswellinwesternblots.Otherapplicationsandotherspecieshavenotyetbeentested. |
BatchNo. | seeviallabel |
Unitsize | 100ug |
Antigen | Aminoacids250-265aa,(FLGNNSGQDIIDFTME)frommouseIL-betaprotein |
Sequence | FLGNNSGQDIIDFTME |
AntigenLocation | C-terminus |
AntigenLength | 15aminoacids |
AntibodyType | RabbitIgG |
Isotype | IgG |
Accession | P10749Here |
Producedin | Rabbit |
CompoundName | Interleukin1beta,IL-1beta |
Purity | 100ugofaffinitypurifiedrabbitIgGin5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3buffer. |
Applications | Westernblot0.5-1.0ug/mL;detectsabandat~17kDaforthematuremouseform.Insomeinstancestheproformat~30kDacanalsobedetected.Thisantibodydoesnotfunctionforimmunohistochemistry. |
Specificity | Mouse,otherspeciesnotyettested.Theantibodymayalsoreactwithratduetosequencehomology. |
SpeciesAgainst | Mouse |
AntibodyAgainst | Mouse |
Cross-reactivity | Otherspeciesnotyettested |
Form | affinitypurified |
Appearance | lyophilized |
Reconstitution | Reconstitutewith200uLofdistilledwater,finalconcentration0.5mg/mL |
Storage | At2-8degCforoneyear(lyophilized).Afterreconstitutionaliquotandstorefrozenat-20degCforuptosixmonths.Avoidrepeatedfreezingandthawing. |
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