Description | GFAPisa50kDaintra-cytoplasmicfilamentousproteinoftheCytoskeletoninastrocytes.Duringthedevelopmentofthecentralnervoussystem,itisacell-specificMarkerthatdistinguishesastrocytesfromotherglialcells.GFAPimmunoreactivityhasbeenshowninimmatureoligodendrocytes,epiglotticcartilage,pituicytes,papillarymeningiomas,myoepithelialcellsofthebreastandinnon-CNS:Schwanncells,salivaryglandneoplasms,entericgliacells,andmetastasizingrenalcarcinomas. |
BatchNo. | Seeproductlabel |
Unitsize | 50µl |
Antigen | RecombinantGFAP(expressedinE.coli)andnativebovineGFAP |
AntibodyType | Antiserum |
OtherNames | Astrocyte;Glialfibrillaryacidicprotein;GFAP; |
Accession | P14136GFAP_HUMAN;Q28115GFAP_BOVIN; |
Producedin | Rabbit |
Applications | WesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:50,000isrecommendedforWB.HumanGFAPhasapredictedlengthof432residuesandaMWof50kDa.Adilutionof1:1000usingfluorescentsecondaryantibodiesor1:5,000usingperoxidaseorotherenzyme-linkedmethodsisrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWB. |
AntibodyAgainst | GlialFibrillaryAcidicProtein |
Cross-reactivity | Human,Rat,Mouse,Feline.Predictedtoreactwithothermammals. |
BlastURL | Clickhere |
Form | Lyophilised |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
References | 1.ReevesS.A,etal.Proc.Natl.Acad.Sci.U.S.A.86:5178-5182(1989). 2.BrennerM,etal.BrainRes.Mol.BrainRes.7:277-286(1990). 2.IsaacsA,etal.Genomics51:152-154(1998). 3.OtaT,etal.Nat.Genet.36:40-45(2004). 4.NielsenA.L,etal.J.Biol.Chem.277:29983-29991(2002). 5.SinghR,etal.Genomics82:185-193(2003). 6.BrennerM,etal.Nat.Genet.27:117-120(2001). 7.BrockmannK,etal.Eur.Neurol.50:100-105(2003). 8.StumpfE,etal.Arch.Neurol.60:1307-1312(2003). 9.SawaishiY,etal.Neurology58:1541-1543(2002). 10.AokiY,etal.Neurosci.Lett.312:71-74(2001). |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。