Description | FUNCTION:Nervegrowthfactorisimportantforthedevelopmentandmaintenanceofthesympatheticandsensorynervoussystems.Itstimulatesdivisionanddifferentiationofsympatheticandembryonicsensoryneurons.SUBUNIT:Homodimer,associatedbynoncovalentforces.SUBCELLULARLOCATION:Secretedprotein.SIMILARITY:BelongstotheNGF-betafamily. |
BatchNo. | Seeproductlabel |
Unitsize | 100µl |
Antigen | NativemousebetaNGFpurifiedfromsuBMAxillarysalivarygland(95%puritybyPAGE) |
OtherNames | Beta-nervegrowthfactor |
Accession | P01139NGF_MOUSE; |
Producedin | Rabbit |
Purity | Wholeserum |
Applications | IHC,1-siteELISA,WB,immunoblot,inhibitionofBIOLOGicalactivity.Adilutionof1:1000-1:5000isrecommendedforIHC,westernblotandimmunoblot;1:15000forELISA;forinhibitionofbiologicalactivity:1:10-50forinvitro,5-10µl/gbodyweightforinvivo.ThisantiserumcompletelyinhibitsneuronalsurvivalandtheoutgrowthactionsofmurineNGFinchickenDRGinvitro.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Acrossreactivityoflessthan1%torecombinanthumanBDNF,NT3,NT4/5byELISAhasbeenshown. |
Cross-reactivity | Thisantiserumisknowntocrossreactwithmouse,rat,humanandavianNGFbotnotbovineNGF. |
Blastit | IfyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteinsfollowthelinkinAccessionfield,selectthenthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
Form | Lyophilised |
Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.MulhallJ.P.etal(2008)JSexMed.May;5(5):1126-36. |
References | 1.Ebendal,T.etal(1989)JNeurosciRes22,223-240. 2.Zhou,X.F.etal(1994)JNeurosciMethods54,95-102. 3.Angeletti,P.U.etal(1968)AdvEnzymolRelatAreasMolBiol31,51-75. 4.HesseK.etal.(1997)NeurosciLett.Aug8;231(2):83-6. 5.MiaoJetal.(2012)NeurosciRes.Dec;74(3-4):269-76. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。