Description | Glyceraldehyde3-PhosphateDehydrogenase(GAPDH)isametabolicenzymeresponsIBLeforcatalyzingonestepintheglycolyticpathway,thereversibleoxidativephosphorylationofglyceraldehyde3-phosphate.GAPDHmayhaveotherrolesintheactivationoftranscriptionandintheregulationofapoptosisaswellasAlzheimer"sdiseaseandHuntington"sdisease.TheimmunogenusedtoraisethisparticularantibodywasextensivelypurifiedpigGAPDH.ThisantibodycanbeusedasaloADIngcontrolforwesternblottingexperiments,allowingcomparisonbetweenthelevelofthisproteinandothersinacellortissue. |
BatchNo. | Seeproductlabel |
Unitsize | 100µL |
Antigen | PurifiedpigGAPDH |
AntibodyType | Polyclonal |
OtherNames | Glyceraldehyde-3-phosphatedehydrogenase;GAPDH;GAPD;G3PDH;GPDH |
Accession | P04406G3P_HUMAN;P00355G3P_PIG; |
Producedin | Rabbit |
Applications | WesternBlotting(WB)andImmunocytochemistry(ICC).Adilutionof1:10,000-1:30,000isrecommendedforWB.HumanGAPDHhasapredictedlengthof335residuesandaMWof36kDa.Adilutionof1:1,000-1:10,000isrecommendedforICC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Specificforthe~36-38kDaGlyceraldehyde3-PhosphateDehydrogenase(GAPDH)protein. |
SpeciesAgainst | Human,Rat,Mouse,Bovine.Itisexpectedthatitwillworkonothermammaltissues. |
BlastURL | Clickhere |
Form | Lyophilisedrabbitserumwith0.5%sodiumazide. |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
GeneralReferences | 1.MorgeneggG,WinklerGC,HubscherU,HeizmannCW,MousJ,KuenzleCC.Glyceraldehyde-3-phosphatedehydrogenaseisanonhistoneproteinandapossibleactivatoroftranscriptioninneurons.JNeuRochem.47:54-6219862.SchulzeH,SchulerA,StuberD,DobeliH,LangernH&HuberG.Ratbrainglyceraldehyde-3-phosphatedehydrogenaseinteractswiththerecombinantcytoplasmicdomainofAlzheimer"sbeta-amyloidprecursorprotein.JNeurochem.60:1915-2219933.BurkeJR,EnghildJJ,MartinME,JouY-S,MyersRM,RosesAD,VanceJM&StrittmatterWJ.HuntingtinandDRPLAproteinsselectivelyinteractwiththeenzymeGAPDH.NatureMed.2:347-350,1996.4.DastoorZ.&Dreyer,J-L.Potentialroleofnucleartranslocationofglyceraldehyde-3-phosphatedehydrogenaseinapoptosisandoxidativestress.J.CellSci.114:1643-16532001.100:429-36(2007). |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。