Description | AGRPistheendogenousantagoNISTofalpha-melanocytestimulatinghormoneandhasbeenshowntocausepotentstimulationoffoodintake,andthisproteinisfoundinover90%ofNeuropeptideYcontainingcellsinrats. |
BatchNo. | Seeproductlabel |
Unitsize | 50µl |
Antigen | Asyntheticpeptide(SPRRCVRLHESCLGQQVPCCDPCATCYCRFFNAFCYCRKLGTATNLCSRT)correspondingtoaregion(82-131)withinthecarboxydomainofmouseagoutirelatedprotein.Thispeptidewasconjugatedtocarrierproteintoenhancetheimmunologicalresponse. |
OtherNames | Agrp;Agrt;Art;Agouti-relatedprotein; |
Accession | AGRP_MOUSE |
Producedin | Guineapig |
Purity | Neatserum |
Applications | IHC,frozen,PFAfixedmaterial.Notyettestedonparaffinembeddedtissuesections.Aconcentrationof1:1000to1:2000isrecommendedforIHCwithovernightincubations.PermeABIlizationsuggestedis0.1%tritonX-100inblockingbuffer.IHCperformedinsheepbrain(hypothalamus)demonstratesintensestainingofcellsandterminals.Nostainingisevidentwhentheprimaryantibodyispre-absorbedwith0.5mg/mlofAGRP.Inrodentssuchasratcellterminalstainingismosttypicallyobservedwithoutcellbodystaining.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Specificitywasdemonstratedbyimmunohistochemistry. |
Cross-reactivity | Thisantibodyisknowntoreactwithhuman,ratandsheep.Otherspecieshavenotyetbeentested. |
Blastit | Ifyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteins,followthelinkintheAccessionfield,selectthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
Form | Lyophilised |
Reconstitution | Reconstitutein50µlsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Itisrecommendedthatathawedsampleisstoredat4ºCfornolongerthan2weeks.Allocationofappropriateanti-bacterialagentcanincreaseshelflifebyseveralweeks.Dilutedserumshouldbepreparedasrequired.Longtermstabilityrequiresstorage,preferablyinsmallaliquotsat-20ºCorlower.Glycerol(1:1)canbeaddedtoneatserumforadditionalstabilityifintendedusedoesnotpreventthis. |
ExpiryDate | 12monthsuponreconstitution |
References | SkrapitsKetal(2014)Colocalizationofcocaine-andamphetamine-regulatedtranscriptwithkisspeptinandneurokininBinthehumaninfundibularregion.PLoSOne9:(8):e103977.PMID:25084101Application:IH,4%PFA,frozensections;1:1000,48hr;Cy5Fluorescentdetectionwithantigenrecovery. GraysonBE,AllenSE,BillesSK,WilliamsSM,SmithMS,GroveKL(2006).Prenataldevelopmentofhypothalamicneuropeptidesystemsinthenonhumanprimate.Neurosci143pp.975-986. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。