![Biosensis/Mouse monoclonal antibody to human CaM kinase 1 [3G1]: IgG/M-810-100/100 µg](images/biosensis/H00008536-WB-HL60 lysate.png)
商品介绍
| Description | CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional regulation, hormone production, translational regulation, regulation of actin filament organisation and neurite outgrowth. CaM kinase I is involved in calcium-dependent activation of the ERK pathway. It phosphorylates proteins with the substrate consensus sequence [MVLIF}-x-R-x(2)-[ST]-x(3)-[MVLIF]. It is activated by calcium ions or calmodulin. It reaches its maximum catalytic activity when phosphorylated by CAMKK1 or CAMKK2. CaM kinase I has a length of 370 amino acids (unprocessed precursor) and a predicted molecular weight of 41.3KDa. It is a monomer that exists in many tissues and is located within the cytoplasm or nucleus. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the CaMK subfamily. |
| Batch No. | See product label |
| Unit size | 100 µg |
| Antigen | Partial recombinant protein of human CaM kinase 1 (amino acids 271 to 370) with a GST tag. |
| Clone | 3G1 |
| Other Names | Calcium/calmodulin-dependent protein kinase type 1; CaM-KI; CaM kinase I alpha; CAMKI-alpha; CAMK1 |
| Accession | KCC1A_HUMAN |
| Produced in | Mouse |
| Purity | Protein G purified immunoglobulin |
| Applications | This antibody is recommended for WB and both direct and sandwich ELISA. This antibody has a detection limit of 0.3ng/ml for recombinant GST tagged CaM kinase 1 when used in sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user. |
| Specificity | The specificity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged CaM Kinase 1 protein. |
| Cross-reactivity | No other species have been tested yet. |
| Blast it | If you would like to see the shared identity between different species or other proteins, follow the link in the Accession field, select the sequence (make sure that you are selecting the sequence that you are interested in, as the sequence may be the precursor rather than the mature protein for example) and copy and paste it HERE and blast/format it. |
| Form | Lyophilised from PBS pH 7.2 |
| Reconstitution | Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. |
| Storage | After reconstitution keep aliquots at -20°C for higher stability or at 4°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles. |
| Expiry Date | 12 months after purchase |
品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。
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