Description | C-CChemokineReceptortype7(CCR-7)isaG-proteincoupledreceptor fortheMIP-3-betachemokine.CCR-7expressionhasbeenfoundinvariouslymphoidtissuesandactivatedB-andT-lymphocytes.CCR-7ishighlyexpressedinB-cellsinfectedwithEpstein-Barrvirus(EBV)andT-cellsinfectedwithherpesvirus6or7. |
BatchNo. | Seeproductlabel |
Unitsize | 100µg |
Antigen | Asyntheticpeptide(FKDLGCLSQEQLRQWSS)correspondingtoaregion(341-357)fromtheC-terminusofhumanC-CChemokineReceptortype7.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA. |
OtherNames | C-Cchemokinereceptortype7;C-CCKR-7;CC-CKR-7;CCR-7;MIP-3betareceptor;EBV-inducedG-proteincoupledreceptor1;EBI1;BLR2;CDw197;CD197; |
Accession | P32248CCR7_HUMAN; |
Producedin | Rabbit |
Purity | Affinitypurifiedonantigencolumn |
Applications | Immunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof1.0-2.0µg/mlisrecommendedforWB.HumanCCR-7(precursor)hasapredictedlengthof378residuesandMWof43kDa.Aconcentrationof1.0-2.0µg/mlisrecommendedtodetecttheproteininformalinfixedandparaffinembeddedtissues.Antigenretrievalisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | ThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen. |
Cross-reactivity | Human;mouse;rat; |
Blastit | Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample. |
Form | Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3 |
Reconstitution | Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial. |
Storage | Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles |
ExpiryDate | 12monthsafterpurchase |
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