商品介绍
| Description | FUNCTION:Energy-transferacceptor.Itsroleistotransducethebluechemiluminescenceoftheproteinaequorinintogreenfluorescentlightbyenergytransfer.FluorescesinvivouponreceivingenergyfromtheCa(2+)-activatedphotoproteinaequorin.BIOPHYSICOCHEMICALPROPERTIES:Excitationmax(nm):488;Emissionmax(nm):509;Extinctioncoefficient(Cm-1M-1):61000.SUBUNIT:Monomer.TISSUESPECIFICITY:Photocytes.PTM:Containsachromophoreconsistingofmodifiedaminoacidresidues.ThechromophoreisformedbyautocatalyticbackbonecondensationbetweenXaa-NandGly-(N+2),andoxidationofTyr-(N+1)todidehydrotyrosine.Maturationofthechromophorerequiresnothingotherthanmolecularoxygen.BIOTECHNOLOGY:Fluorescentproteinshavebecomeausefulandubiquitoustoolformakingchimericproteins,wheretheyfunctionasafluorescentproteintag.TypicallytheytolerateN-andC-terminalfusiontoabroadvarietyofproteins.TheyhavebeenexpressedinmostknowncelltypesandareusedasanoninvasivefluorescentMarkerinlivingcellsandorganisms.Theyenableawiderangeofapplicationswheretheyhavefunctionedasacelllineagetracer,reporterofgeneexpression,orasameasureofprotein-proteininteractions.SIMILARITY:BelongstotheGFPfamily. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µl |
| Antigen | RecombinantEGFP |
| OtherNames | RecombinantEnhancedGreenFluorescenceProtein |
| Accession | EGFPprotein GFP_AEQVI |
| Producedin | Rabbit |
| Purity | Wholeserum |
| Applications | IHC,Westernblot.Recommendedtobeusedatadilutionof1:1000-1:5000forbothapplications.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | ThisantibodyisknowntoreactwithEGFPconfirmedbyIHCandWB. |
| Blastit | IfyouwouldliketoseethesharedidentitybetweendifferentspeciesorotherproteinsfollowthelinkinAccessionfield,selectthenthesequence(makesurethatyouareselectingthesequencethatyouareinterestedin,asthesequencemaybetheprecursorratherthanthematureproteinforexample)andcopyandpasteitHEREandblast/formatit. |
| Form | Lyophilised |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
| Storage | Afterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchase |
| References | 1.Chalfie,M.,Tu,Y.etal(1994)Science263,802-805 2.Tsien,R.Y.(1998)AnnuRevBiochem67,509-544 3.Dardalhon,V.etal(1999)HumGeneTher10,5-14 4.Kain,S.R.(1999)DDT4,304-312 |
品牌介绍
Biosensis专注于神经科学领域的抗体和试剂的开发，特别是神经营养因子和神经营养因子受体。 近30年来，Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子，我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症（AD）、帕金森氏症（PD）和肌萎缩侧索硬化（ALS）疾病，以及自噬和代谢应激障碍，包括肥胖、代谢综合征的研究、神经免疫学和炎症。
公司介绍
产品分类
TR-200-BG
PE-1243-50
Antibody Kits
PE-1246-5
TR-100-BG
S-1754-500
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Diabetes & Obesity
Intracellular Trafficking
PE-1239-5
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B-100-PS
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2 Plate ELISA kits
M-1763-100-FT
TR-200-BGT
PE-1239-2
PE-1242-5
M-1249-100
Neurotrophic Factor Receptors
Cell Cycle Regulators
S-1759-100
PE-1606-100
PE-1762-100
B-200-PS
PE-019-100
PE-1241-5
PE-003-100
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BL-003-1000
PE-1246-2
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PE-1253-100
TR-100-FJ
BL-005-500
Neuroendocrine
M-1728-100
DNA Damage & Repair
PE-001-100
PE-1608-1
TR-400-AG
PE-1243-25
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PE-1608-5
TR-100-FJT
Autophagy
PE-1242-2
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Tag Antibodies
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PE-019-25
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BL-002-1250
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Signalling & Transcription
Matrix & Adhesion Molecules
BL-001-1250
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PE-019-500
Stem Cells
Junction Proteins
TR-300-AG
Apoptosis
PE-1252-100
Growth Factors
Development
Alzheimer\'s / Parkinson\'s
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