Description | Neurofilamentsarecomposedofthreeintermediatefilamentproteins:light(~68kDa),medium(~160kDa)andheavy(~200kDa),whichareinvolvedinthemaintenanceoftheneuronalcaliber.Neurofilamentlight(NF68orNF-L)isthemostabundantofthethreeproteins. |
BatchNo. | Seeproductlabel |
Unitsize | 50µl |
Antigen | ApreparationofenzymaticallydephosphorylatedpigneurofilamentsincludingNF-L,NF-MandNF-H.ScreeningwasbyELISAontheimmungenfollowedbyimmunofluorescencemicroscopy.TheepitopeforthisantibodyisnotintheN-terminusbutitsexactlocationisnotknown.Itisthoughttobesomewherewithinthecentralalphahelical"rod"regionofthemolecule. |
AntibodyType | Monoclonal |
Isotype | IgG1 |
Clone | DA2 |
OtherNames | NF-L;NF68;NEFL;Neurofilamentlightpolypeptide;NFL; |
Accession | P02547NFL_PIG;P07196NFL_HUMAN; |
Producedin | Mouse |
Applications | WesternBlotting(WB),Immunocytochemistry(IC),Immunohistochemistry(IH)andFlowCytometry(2ugper10^6cells).Adilutionof1:10,000-1:20,000isrecommendedforWB.Adilutionof1:100-1:500isrecommendedforICandIH.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | SpecificallyrecognizesthelightneurofilamentsubunitNF-L(~68kDa)inWB. |
AntibodyAgainst | NeurofilamentLight |
Cross-reactivity | Hu,Rat,Ms,Fel,Bov,Por,Chk |
BlastURL | Clickhere |
Form | Lyophilisedwith5%trehalose |
Appearance | Whitepowder |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
SpecificReferences | 1.FelitsynN.etal(2008)Thehemeprecursordelta-aminolevulinateblocksperipheralmyelinformation.JNeuRochem.2008Sep;106(5):2068-79. |
Biosensis专注于神经科学领域的抗体和试剂的开发,特别是神经营养因子和神经营养因子受体。 近30年来,Biosensis一直是该领域的全球领航者和OEM供应商。除神经营养因子,我们的神经科学产品组合还被广泛用于神经退行性疾病、神经发育和神经代谢的研究。重点研究领域包括阿尔茨海默氏症(AD)、帕金森氏症(PD)和肌萎缩侧索硬化(ALS)疾病,以及自噬和代谢应激障碍,包括肥胖、代谢综合征的研究、神经免疫学和炎症。